Preparation of Active Chitooligosaccharides with a Novel Chitosanase AqCoA and Their Application in Fungal Disease Protection
文献类型： 外文期刊
作者： Wang, Yanxin 1 ; Li, Ding 2 ; Liu, Muxing 3 ; Xia, Chengyao 1 ; Fan, Qiwen 1 ; Li, Xu 1 ; Lan, Zejun 1 ; Shi, Guolong 1 ; Do 1 ;
作者机构： 1.Nanjing Agr Univ, Key Lab Agr Environm Microbiol, Minist Agr & Rural Affairs, Coll Life Sci, Nanjing 210095, Peoples R China
2.Jiangsu Acad Agr Sci, Inst Vet Immunol & Engn, Nanjing 210014, Peoples R China
3.Nanjing Agr Univ, Coll Plant Protect, Key Lab Monitoring & Management Plant Dis & Insec, Chinese Minist Agr, Nanjing 210095, Peoples R China
4.Nanjing Tech Univ, Coll Biotechnol & Pharmaceut Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 211800, Peoples R China
关键词： chitosanase; chitosan oligosaccharides; controllable hydrolysis; antifungal properties; plant immunity activation; biocontrol agent
期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：4.192； 五年影响因子：4.289 ）
ISSN： 0021-8561
年卷期： 2021 年 69 卷 11 期
页码：
收录情况： SCI
摘要： Enzymes that degrade fungal cell walls and the resulting oligosaccharides are promising weapons to combat plant fungal disease. In this study, we identified a novel endo-chitosanase, AqCoA, from Aquabacterium sp. A7-Y. The enzyme showed a specific activity of 18 U/mg toward 95% deacetylated chitosan at pH 5.0 and 40 degrees C. AqCoA also showed activity toward sodium carboxymethylcellulose, indicating substrate promiscuity. AqCoA hydrolyzed chitosan into chitooligosaccharides (CoA-COSs) with degrees of polymerization (DPs) of 3-5 but showed no activity toward CoA-COSs with DPs <6, indicating an endo-type activity. At 2.5 mu g/mL, AqCoA inhibited appressorium formation of Magnaporthe oryzae; the produced CoA-COSs also inhibited the growth of M. oryzae and Fusarium oxysporum. Furthermore, CoA-COSs acted as immune elicitors in rice by inducing the reactive oxygen species burst and the expression of defense genes. These results demonstrated that AqCoA and its resulting CoA-COSs might be effective tools for protecting plants against pathogenic fungi.
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