文献类型： 外文期刊

作者： Lin, Zezhong ¹ ; Guo, Cuiting ¹ ; Lou, Shuaitong ¹ ; Jin, Songsong ¹ ; Zeng, Weike ¹ ; Guo, Yanan ¹ ; Fang, Jun ³ ; Xu, Z ¹ ;

作者机构： 1.Fujian Agr & Forestry Univ, Coll Forestry, Basic Forestry & Prote Res Ctr, Fuzhou 350002, Peoples R China

2.Fujian Agr & Forestry Univ, Coll Life Sci, Fujian Prov Key Lab Plant Funct Biol, Fuzhou 350002, Peoples R China

3.Shanghai Acad Agr Sci, Crop Breeding & Cultivating Inst, Shanghai 201403, Peoples R China

4.Guangxi Forestry Res Inst, Guangxi Key Lab Super Timber Trees Resource Culti, Nanning 530002, Peoples R China

5.Jilin Univ, Coll Plant Sci, Jilin Prov Engn Lab Plant Genet Improvement, Changchun 130062, Peoples R China

关键词： PeNLPs; Transcription factor; Gene expression; Nitrate signaling; Phyllostachys edulis

期刊名称：PLANT SCIENCE （ 影响因子：3.591； 五年影响因子：4.253 ）

ISSN： 0168-9452

年卷期： 2021 年 306 卷

页码：

收录情况： SCI

摘要： For rapid growth, moso bamboo (Phyllostachys edulis) requires large amounts of nutrients. Nitrate is an indispensable molecular signal to regulate nitrogen absorption and assimilation, which are regulated by group III NINLIKE PROTEINs (NLPs). However, no Phyllostachys edulis NLP (PeNLP) has been characterized. Here, eight PeNLPs were identified, which showed dynamic expression patterns in bamboo tissues. Nitrate did not affect PeNLP mRNA levels, and PeNLP1,-2,-5,-6,-7, and-8 successfully restored nitrate signaling in Arabidopsis atnlp71 protoplasts through recovering AtNiR and AtNRT2.1 expression. Four group I and II PeNLPs (PeNLP1,-2,-5, and-8) interacted with the nitrate-responsive cis-element of PeNiR. Moreover, nitrate triggered the nuclear retention of PeNLP8. PeNLP8 overexpression in Arabidopsis significantly increased the primary root length, lateral root number, leaf area, and dry and wet weight of the transgenic plants, and PeNLP8 expression rescued the root architectural defect phenotype of atnlp7-1 mutants. Interestingly, PeNLP8 overexpression dramatically reduced nitrate content but elevated total amino acid content in Arabidopsis. Overall, the present study unveiled the potential involvement of group I and II NLPs in nitrate signaling regulation and provided genetic resources for engineering plants with high nitrogen use efficiency.