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Creating a novel herbicide-tolerance OsALS allele using CRISPR/Cas9-mediated gene editing

文献类型： 外文期刊

作者： Wang, Fangquan 1 ; Xu, Yang 1 ; Li, Wenqi 1 ; Chen, Zhihui 1 ; Wang, Jun 1 ; Fan, Fangjun 1 ; Tao, Yajun 1 ; Jiang, Yanji 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing Branch, Chinese Natl Ctr Rice Improvement, Nanjing 210014, Jiangsu, Peoples R China

2.Yangzhou Univ, Jiangsu Coinnovat Ctr Modern Prod Technol Grain C, Yangzhou 225009, Jiangsu, Peoples R China

3.Jiangsu Acad Agr Sci, Prov Key Lab Agrobiol, Nanjing 210014, Jiangsu, Peoples R China

4.CSIRO Agr & Food, GPO Box 1700, Canberra, ACT 2601, Australia

5.Jiangsu Univ, Inst Life Sci, Zhenjiang 212013, Jiangsu, Peoples R China

关键词： Rice; CRISPR/Cas9; ALS; Herbicide-tolerance; Crop breeding

期刊名称：CROP JOURNAL （ 影响因子：3.395； ）

ISSN： 2095-5421

年卷期： 2021 年 9 卷 2 期

页码：

收录情况： SCI

摘要： Weeds and weedy rice plague commercial rice fields in many countries. Developing herbicide-tolerance rice is the most efficient strategy to control weed proliferation. CRISPR/Cas9-mediated gene editing, which generates small InDels and nucleotide substitutions at and around target sites using error-prone non-homologous end joining DNA repairing, has been widely adopted for generation of novel crop germplasm with a wide range of genetic variation in important agronomic traits. We created a novel herbicide-tolerance allele in rice by targeting the acetolactate synthase (OsALS) gene using CRISPR/Cas9-mediated gene editing. The novel allele (G628W) arose from a G-to-T transversion at position 1882 of OsALS and conferred a high level of herbicide tolerance. Transgene-free progeny carrying homozygous G628W allele were identified and showed agronomic performance similar to that of wild-type plants, suggesting that the G628W allele is a valuable resource for developing elite rice varieties with strong herbicide tolerance. To promote use of the G628W allele and to accelerate introgression and/or pyramiding of the G628W allele with other elite alleles, we developed a DNA marker for the G628W allele that accurately and robustly distinguished homozygous from heterozygous segregants. Our result further demonstrates the feasibility of CRISPR/Cas9-mediated gene editing in creating novel genetic variation for crop breeding. (C) 2020 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

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