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The synergistic effects of phenyllactic acid and slightly acid electrolyzed water to effectively inactivate Klebsiella oxytoca planktonic and biofilm cells

文献类型： 外文期刊

作者： Liu, Fang 1 ; Tang, Chun 1 ; Wang, Debao 1 ; Sun, Zhilan 1 ; Du, Lihui 3 ; Wang, Daoying 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Inst Agr Prod Proc, Nanjing 210014, Peoples R China

2.Jiangsu Univ, Sch Food & Biol Engn, Zhenjiang 212013, Jiangsu, Peoples R China

3.Nanjing Univ Finance & Econ, Coll Food Sci & Engn, Collaborat Innovat Ctr Modern Grain Circulat & Sa, Key Lab Grains & Oils Qual Control & Proc, Nanjing 210023, Peoples R China

关键词： Phenyllactic acid; Slightly acid electrolyzed water; Biofilm; Polysaccharide; ATP

期刊名称：FOOD CONTROL （ 影响因子：4.258； 五年影响因子：4.421 ）

ISSN： 0956-7135

年卷期： 2021 年 125 卷

页码：

收录情况： SCI

摘要： 3-Phenyllactic acid (PLA) and slightly acid electrolyzed water (SAEW) are effective antimicrobial agents with broad-spectrum activity. The combined effects of PLA and SAEW on the inactivation of Klebsiella oxytoca planktonic and biofilm cells were evaluated in this study. The K. oxytoca K6 planktonic and biofilm cells were treated with PLA (0.5%, 1% and 2%), SAEW (30 and 60 mg/L), 0.5% PLA plus 30 mg/L SAEW, and 1% PLA plus 30 mg/L SAEW for 5, 10, 20, 30, and 60 min. The planktonic and biofilm cell assays showed that 2% PLA was more effective for the inactivation of K. oxytoca planktonic and biofilm cells than the other agents, and the combination of PLA and SAEW was more effective than the single agents at the same concentrations. Confocal laser scanning microscopy and ATP leakage analysis showed that the combined treatment of PLA and SAEW at different concentrations destroyed the cell membrane integrity of K. oxytoca planktonic and biofilm cells and caused ATP leakage at different levels. Scanning electron microscopy (SEM) assays showed that the morphologies of K. oxytoca planktonic and biofilm cells changed after treatment with the antibacterial agents at different levels. The cells were seriously damaged after treatment with 1% PLA and 30 mg/L SAEW for 60 min. The biofilm polysaccharide detection assays showed that SAEW-containing antibacterial agents were effective for disrupting the biofilm extracellular polymeric matrix, as shown in SEM images. The combination of PLA and SAEW effectively inactivated K. oxytoca planktonic and biofilm cells by damaging the cell membrane and disrupting the biofilm matrix.

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