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Deep Sequencing, Nested PCR, and Denaturing Gradient Gel Electrophoresis Reveal a Wider Distribution of Chinese Caterpillar Mushroom, Ophiocordyceps sinensis (Ascomycetes), in Native Soil Types

文献类型： 外文期刊

作者： Yang, Rui-Heng 1 ; Wang, Xiao-Liang 1 ; Wang, Ke 1 ; Hsiang, Tom 4 ; Wang, Wen-Jing 1 ; Wu, Hai-Jun 1 ; Li, Yi 1 ; Li, Xi 1 ;

作者机构： 1.Chinese Acad Sci, Inst Microbiol, State Key Lab Mycol, Beijing 100101, Peoples R China

2.Shanghai Acad Agr Sci, Inst Edible Fungi, Shanghai 201403, Peoples R China

3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

4.Univ Guelph, Sch Environm Sci, Guelph, ON N1G 2W1, Canada

5.Fujian Agr & Forestry Univ, Coll Plant Protect, Fuzhou 350002, Peoples R China

关键词： Ophiocordyceps sinensis; distribution; pyrosequencing; DGGE; nested PCR; medicinal mushrooms

期刊名称：INTERNATIONAL JOURNAL OF MEDICINAL MUSHROOMS （ 影响因子：1.423； 五年影响因子：1.525 ）

ISSN： 1521-9437

年卷期： 2021 年 23 卷 4 期

页码：

收录情况： SCI

摘要： Ophiocordyceps sinensis appears as stroma emerging from underground sclerotium enclosed by the skeleton of Thitarodes moth larvae. However, the actual distribution of the fungus in soil still remains unclarified. In this study, 40 soil samples were used for detection of O. sinensis to confirm its distribution in native habitats using denaturing gradient gel electrophoresis, nested internal transcribed spacer (ITS) PCR, and 454 pyrosequencing methods. The soil samples included six types: Os, where both stromata and host moth larvae were found; NL, representing no signs of stromata, but where moth larvae were found; NOs, where neither stroma nor moth larvae were found; BS, with bare soil without the presence of stroma of O. sinensis or moth larvae; AF, from soil surrounding the stroma; and MP, soil particles firmly wrapping the sclerotium of O. sinensis. Of 40 samples tested, 36 showed positive detection of O. sinensis by at least one of the three detection methods, with positive detection in all six sample types at all five sites. The results showed that traces of O. sinensis can be detected in locations with no macroscopically visible evidence of the fungus or its host and at least 100 m away from such locations.

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