您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Roles of bulk and rhizosphere denitrifying bacteria in denitrification from paddy soils under straw return condition

文献类型： 外文期刊

作者： Wang, Ning 1 ; Zhao, Ya-Hui 1 ; Yu, Jian-Guang 1 ; Xue, Li-Hong 1 ; Li, Hong-Bo 1 ; Yang, Lin-Zhang 1 ;

作者机构： 1.Jiangsu Acad Agr Sci, Key Lab Agroenvironm Downstream Yangtze Plain, Minist Agr & Rural Affairs Peoples Republ China, Nanjing 210014, Jiangsu, Peoples R China

2.Jiangsu Univ, Sch Environm & Safety Engn, Zhenjiang 212001, Jiangsu, Peoples R China

3.Anhui Sci & Technol Univ, Coll Urban Construct & Environm Sci, Fengyang 233100, Anhui, Peoples R China

4.Nanjing Univ, Sch Environm, State Key Lab Pollut Control & Resource Reuse, Nanjing, Peoples R China

关键词： Denitrifying bacteria; Rhizosphere and bulk; Straw return; Denitrification; Paddy soils

期刊名称：JOURNAL OF SOILS AND SEDIMENTS （ 影响因子：2.763； 五年影响因子：2.998 ）

ISSN： 1439-0108

年卷期：

页码：

收录情况： SCI

摘要： Purpose Roles of bulk and rhizosphere denitrifying bacteria in paddy soil denitrification under straw return conditions are rarely discriminated, limiting our understanding on nitrogen biogeochemistry in soils amended with straw. The objective of this study was to explore the microbial mechanisms accounting for the altered rhizosphere and bulk soil denitrification with straw amendment. Methods In this study, straw was added into two representative paddy soils (0.5% w/w) from Yixing (YX) and Taizhou (TZ) in Jiangsu city, China, for rice growth over a 37-day period. Denitrification potentials (net N2O emission, total denitrification, and N2O reduction rates) of rhizosphere and bulk soils at the end of rice growth were measured using an acetylene inhibition technique. In addition, denitrifying bacterial community compositions and functional gene abundances were analyzed using Illumina sequencing and quantitative real-time PCR. Results and discussion For control treatment without straw addition, total denitrification and N2O reduction rates were significantly higher in the rhizosphere than that in bulk soils; however, net N2O emission potentials were similar between rhizosphere and bulk for TZ (22.6-53.3 vs. 9.44-46.6 mg N kg(-1)) and YX (946 +/- 126 vs. 699 +/- 350 mg N kg(-1)). Under straw return condition, N2O emission potentials from the bulk of both soils were significantly elevated, corresponding to the increase in the relative abundance of some taxa of denitrifying bacteria (unnamed environmental samples and unclassified proteobacteria) and increase in nirK, nirS, and nosZ genes. This indicates a positive response of bulk soil denitrifying bacteria to straw addition. In contrast, straw addition produced inhibitive effects on the growth of denitrifying bacteria with decreased nirK, nirS, and nosZ gene abundances in the rhizosphere. This led to decreased N2O emission potentials at the end of incubation for rhizosphere soils, which were 98.2% and 25.2% lower for TZ and YX compared to those of bulk soils. Conclusions These results suggested that the roles of bulk and rhizosphere denitrifying bacteria in paddy soil denitrification shifted with straw addition, which might inspire further studies to target the denitrification hotspots to effectively mitigate greenhouse emissions at the early period of rice growth.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台