文献类型： 外文期刊

作者： Yang, Yu-Hong ¹ ; Hao, Yi-Ming ² ; Liu, Xiao-Fang ³ ; Gao, Xiang ⁴ ; Wang, Bao-Zhen ² ; Takahashi, Koretaro ⁵ ; Du, Le ¹ ;

作者机构： 1.Qilu Univ Technol, Shandong Acad Sci, Sch Food Sci & Engn, 3501 Daxue Rd, Jinan 250353, Shandong, Peoples R China

2.Shandong Univ, Sch Publ Hlth, Cheeloo Coll Med, Dept Nutr & Food Hyg, 44 Wenhuaxi Rd, Jinan 250012, Shandong, Peoples R China

3.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Key Lab Sustainable Dev Polar Fishery, Minist Agr & Rural Affairs, 106 Nanjing Rd, Qingdao 266071, Shandong, Peoples R China

4.Qingdao Univ, Coll Life Sci, 308 Ningxia Rd, Qingdao 266071, Shandong, Peoples R China

5.Kitami Inst Technol, Fac Engn, 165 Koen Cho, Kitami, Hokkaido 0908507, Japan

期刊名称：FOOD & FUNCTION （ 影响因子：4.171； 五年影响因子：4.364 ）

ISSN： 2042-6496

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收录情况： SCI

摘要： Some chronic diseases such as cancer-associated cachexia (CAC) and obesity are associated with the overproduction of tumor necrosis factor-alpha (TNF-alpha) that stimulates excess lipolysis in adipocytes. Our previous studies have shown that docosahexaenoic acid-enriched phospholipids (DHA-PL) and eicosapentaenoic acid-enriched phospholipids (EPA-PL) ameliorated CAC and obesity-related metabolic disorders. To identify the molecular mechanisms involved, we examined the impact and the associated signaling pathways of DHA-PL and EPA-PL on TNF-alpha-induced lipolysis in 3T3-L1 adipocytes. The present results revealed that DHA-PL and EPA-PL inhibited the TNF-alpha-induced increase of glycerol release and protected lipid droplets. In addition, DHA-PL and EPA-PL increased DHA and EPA contents in the phospholipid fraction of adipocytes, respectively. Moreover, DHA-PL and EPA-PL enhanced sirtuin 1 (SIRT1) deacetylase activity and its protein expression. By activating SIRT1, DHA-PL and EPA-PL upregulated the G0/G1 switch gene 2 protein level to inhibit adipose triglyceride lipase activity, activate AMP-activated protein kinase to reverse the downregulation of perilipin expression and phosphorylation of hormone-sensitive lipase (HSL) at Ser565 and prevent the phosphorylation of HSL at Ser660. Furthermore, DHA-PL and EPA-PL improved glucose uptake and glucose transporter type 4 translocation to the plasma membrane in TNF-alpha-treated adipocytes. Thus, it was concluded that DHA-PL and EPA-PL inhibit TNF-alpha-induced lipolysis in 3T3-L1 adipocytes by activating the SIRT1 pathways.