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Isolation, purification and the anti-hypertensive effect of a novel angiotensin I-converting enzyme (ACE) inhibitory peptide from Ruditapes philippinarum fermented with Bacillus natto

文献类型： 外文期刊

作者： Chen, Yingyun 1 ; Gao, Xiang 1 ; Wei, Yuxi 1 ; Liu, Qi 2 ; Jiang, Yuhong 3 ; Zhao, Ling 2 ; Ulaah, Sadeeq 4 ;

作者机构： 1.Qingdao Univ, Coll Life Sci, Qingdao 266071, Peoples R China

2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Qingdao 266071, Peoples R China

3.Qingdao Women & Children Med Ctr, Qingdao 266035, Peoples R China

4.Beijing Univ Chem Technol, Coll Life Sci, Beijing 100029, Peoples R China

期刊名称：FOOD & FUNCTION （ 影响因子：5.396； 五年影响因子：5.534 ）

ISSN： 2042-6496

年卷期： 2018 年 9 卷 10 期

页码：

收录情况： SCI

摘要： Hypertension is known as an important factor in cardiovascular disease. An angiotensin-I-converting enzyme (ACE) inhibitor is one of the synthetic drugs that is widely used to control hypertension. Here, we have prepared and investigated the ACE inhibitory peptide from Ruditapes philippinarum fermented with Bacillus natto. The fermented product obtained under optimized fermentation conditions exhibited ACE inhibitory activity. The ACE inhibitory peptides were purified by ultrafiltration, gel filtration chromatography and RP-HPLC, sequentially. A novel peptide with high ACE inhibitory activity (IC50 of 8.16 M) was isolated and the amino acid sequence was identified to be Val-Ile-Ser-Asp-Glu-Asp-Gly-Val-Thr-His (VISDEDGVTH) by LC-MS/MS. The Lineweaver-Burk plots suggested that VISDEDGVTH acts as a competitive inhibitor against ACE. Our in vitro study indicated the stability of VISDEDGVTH against gastrointestinal proteases. An in vivo study in rats proved the significant anti-hypertension effect of the peptide. Furthermore, the cellular mechanism of VISDEDGVTH moderating hypertension was investigated. We found that VISDEDGVTH enhanced the release of NO, inhibited the secretion of EF-1, and scavenged ROS in human vascular endothelial cells. Our study suggests that VISDEDGVTH serves as an anti-hypertension and anti-inflammatory drug and as a beneficial ingredient in functional foods and pharmaceuticals.

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