Determination of tetrodotoxin in puffer fish by ion-paired reagent-assisted dispersive solid-phase extraction (ion-paired-dSPE) in combination with hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS)
文献类型： 外文期刊
作者： Hou, Chao-Ping 1 ; Huang, Ke 1 ; Wang, Qiang 1 ; Yang, Jin-Lan 5 ; Chen, Hui-Bing 6 ; Zhang, Ying-Xia 1 ; Liu, Qi 1 ; Li, Zhi-Guang 1 ; Li, Hui-Qing 1 ; Wang, Xu-Feng 1 ;
作者机构： 1.Chinese Acad Fishery Sci, South China Sea Fisheries Res Inst, Guangzhou, Peoples R China
2.Sanya Trop Fisheries Res Inst, Sanya 572025, Hainan, Peoples R China
3.Minist Agr & Rural Affairs, Fishery Environm & Aquat Prod Qual Testing Ctr, Guangzhou 510300, Peoples R China
4.Guangdong Prov Key Lab Fishery Ecol & Environm, Guangzhou 510300, Peoples R China
5.Guangzhou Acad Agr & Rural Sci, Guangzhou 510300, Peoples R China
6.Guangzhou Nansha Mingxi Inspection & Testing Serv, Guangzhou 510300, Peoples R China
关键词： Ion-paired reagent assisted dispersive solid phase extraction (Ion-paired-dSPE); Hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/MS); Tetrodotoxin (TTX); Puffer fish
期刊名称：MICROCHEMICAL JOURNAL （ 影响因子：5.1； 五年影响因子：4.7 ）
ISSN： 0026-265X
年卷期： 2025 年 212 卷
页码：
收录情况： SCI
摘要： Tetrodotoxin (TTX), one of the most potent neurotoxins, poses a significant threat to consumer health. This study presents an advanced analytical method combining ion-paired reagent-assisted dispersive solid-phase extraction (ion-paired-dSPE) with hydrophilic interaction liquid chromatography-tandem mass spectrometry (HILIC-MS/ MS), for the quantification of TTX residues in puffer fish. Edible tissue samples were extracted twice using a 1 % formic acid-methanol solution and purified via optimized ion-paired-dSPE procedures. TTX adsorbed onto the magnetic nano-adsorbents was subsequently eluted with an acetonitrile-water-formic acid solution (60:40:1, v/ v/v). Detection was achieved through electrospray ionization in positive mode, with quantification performed in multiple reaction monitoring (MRM) mode. Correlation coefficients (r) in matrix-matched external standard curves ranged from 0.9995 to 0.9997. The limits of detection (LOD) and quantification (LOQ) in puffer fish tissues were 0.1 mu g/kg and 0.3 mu g/kg, respectively. Spiked recoveries at three concentrations in negative tissue samples ranged from 73.4 % to 87.5 %, with precision under 10.6 %. The method was validated in accordance with 2002/657/EC guidelines and successfully applied to TTX detection in cultured puffer fish samples.
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