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Sulfitobacter alexandrii sp. nov., a new microalgae growth-promoting bacterium with exopolysaccharides bioflocculanting potential isolated from marine phycosphere

文献类型： 外文期刊

作者： Yang, Qiao 1 ; Ge, Ya-ming 3 ; Iqbal, Nurhezreen Md 4 ; Yang, Xi 5 ; Zhang, Xiao-ling 1 ;

作者机构： 1.Zhejiang Ocean Univ, Dept Marine Chem, Zhoushan, Peoples R China

2.Zhejiang Ocean Univ, ABI Grp GPM Project, Zhoushan, Peoples R China

3.Natl Engn Res Ctr Marine Aquaculture, Zhoushan, Peoples R China

4.Natl Inst Biotechnol Malaysia, Malaysia Genome Inst, Kajang, Malaysia

5.Guangdong Acad Agr Sci, Inst Anim Sci, Guangzhou, Peoples R China

关键词： Algae-bacteria interactions; Phycosphere microbiota; Sulfitobacter alexandrii; Alexandrium minutum; Microalgae growth-promoting bacterium; Paralytic shellfish poisoning toxins

期刊名称：ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY （ 影响因子：2.674； 五年影响因子：2.09 ）

ISSN： 0003-6072

年卷期：

页码：

收录情况： SCI

摘要： Marine phycosphere harbors unique cross-kingdom associations with enormous ecological significance in aquatic ecosystems as well as relevance for algal biotechnology industry. During our investigating the microbial composition and bioactivity of marine phycosphere microbiota (PM), a novel lightly yellowish and versatile bacterium designated strain AM1-D1(T) was isolated from cultivable PM of marine dinoflagellate Alexandrium minutum amtk4 that produces high levels of paralytic shellfish poisoning toxins (PSTs). Strain AM1-D1(T) demonstrates notable bioflocculanting bioactivity with bacterial exopolysaccharides (EPS), and microalgae growth-promoting (MGP) potential toward its algal host. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain AM1-D1(T) was affiliated to the members of genus Sulfitobacter within the family Rhodobacteraceae, showing the highest sequence similarity of 97.9% with Sulfitobacter noctilucae NB-68(T), and below 97.8% with other type strains. The complete genome of strain AM1-D1(T) consisted of a circular 3.84-Mb chromosome and five circular plasmids (185, 95, 15, 205 and 348 Kb, respectively) with the G+C content of 64.6%. Low values obtained by phylogenomic calculations on the average nucleotide identity (ANI, 77.2%), average amino acid identity (AAI, 74.7%) and digital DNA-DNA hybridization (dDDH, 18.6%) unequivocally separated strain AM1-D1(T) from its closest relative. The main polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, diphosphatidylglycerol, one unidentified phospholipid and one unidentified lipid. The predominant fatty acids (> 10%) were C-18:1 omega 7c, C-19:0 cyclo omega 8c and C-16:0. The respiratory quinone was Q-10. The genome of strain AM1-D1(T) was predicted to encode series of gene clusters responsible for sulfur oxidation (sox) and utilization of dissolved organic sulfur exometabolites from marine dinoflagellates, taurine (tau) and dimethylsulfoniopropionate (DMSP) (dmd), as well as supplementary vitamin B-12 (cob), photosynthesis carotenoids (crt) which are pivotal components during algae-bacteria interactions. Based on the evidences by the polyphasic characterizations, strain AM1-D1(T) represents a novel species of the genus Sulfitobacter, for which the name Sulfitobacter alexandrii sp. nov. is proposed. The type strain is AM1-D1(T) (= CCTCC 2017277T = KCTC 62491(T)).

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