Transcriptome profile analysis reveals KLHL30 as an essential regulator for myoblast differentiation
文献类型： 外文期刊
作者： Chen, Genghua 1 ; Yin, Yunqian 1 ; Lin, Zetong 1 ; Wen, Huaqiang 1 ; Chen, Jiahui 1 ; Luo, Wen 1 ;
作者机构： 1.South China Agr Univ, Coll Anim Sci, Dept Anim Genet Breeding & Reprod, Guangzhou 510642, Guangdong, Peoples R China
2.South China Agr Univ, Guangdong Prov Key Lab Agroanim Genom & Mol Breed, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
3.South China Agr Univ, Key Lab Chicken Genet Breeding & Reprod, Minist Agr & Rural Affair, Guangzhou 510642, Peoples R China
关键词： KLHL30; Differentially expressed genes; Proliferation; Myogenesis; Differentiation; Myoblast
期刊名称：BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS （ 影响因子：2.985； 五年影响因子：2.75 ）
ISSN： 0006-291X
年卷期： 2021 年 559 卷
页码：
收录情况： SCI
摘要： Skeletal muscle development is a sophisticated multistep process orchestrated by diverse myogenic transcription factors. Recent studies have suggested that Kelch-like genes play vital roles in muscle disease and myogenesis. However, it is still unclear how Kelch-like genes impact myoblast physiology. Here, through integrative analysis of the mRNA expression profile during chicken primary myoblast and C2C12 differentiation, many differentially expressed genes were found and suggested to be enriched in myoblast differentiation and muscle development. Interestingly, a little-known Kelch-like gene KLHL30 was screened as skeletal muscle-specific gene with essential roles in myogenic differentiation. Tran-scriptomic data and quantitative PCR analysis indicated that the expression of KLHL30 is upregulated under myoblast differentiation state. KLHL30 overexpression upregulated the protein expression of myogenic transcription factors (MYOD, MYOG, MEF2C) and induced myoblast differentiation and myotube formation, while knockdown of KLHL30 caused the opposite effect. Furthermore, KLHL30 was found to significantly decrease the numbers of cells in the S stage and thereby depress myoblast proliferation. Collectively, this study highlights that KLHL30 as a muscle-specific regulator plays essential roles in myoblast proliferation and differentiation. ? 2021 Elsevier Inc. All rights reserved.
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