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Tobacco rattle virus-induced gene silencing in Hevea brasiliensis

文献类型： 外文期刊

作者： Li, Hui-Liang 1 ; Guo, Dong 1 ; Wang, Ying 1 ; Zhu, Jia-Hong 1 ; Qu, Long 1 ; Peng, Shi-Qing 1 ;

作者机构： 1.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Key Lab Biol & Genet Resources Trop Crops, Minist Agr, Haikou, Hainan, Peoples R China

2.Hainan Univ, Sch Life & Pharmaceut Sci, Haikou, Hainan, Peoples R China

3.Hainan Acad Trop Agr Resource, CATAS, Haikou, Hainan, Peoples R China

关键词： Hevea brasiliensis; phytoene desaturase; tobacco rattle virus; virus-induced gene silencing

期刊名称：BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY （ 影响因子：1.516； 五年影响因子：1.542 ）

ISSN： 0916-8451

年卷期： 2021 年 85 卷 3 期

页码：

收录情况： SCI

摘要： Virus-induced gene silencing (VIGS) is a powerful gene-silencing tool that has been intensively applied in plants. To data, the application of VIGS in rubber tree has not yet been reported. In this study, we described the efficient gene silencing in rubber tree by VIGS. The gene encoding Hevea brasiliensis phytoene desaturase (HbPDS) was identified in rubber tree genome. Small interfering RNAs from HbPDS and the silencing gene fragment were predicted and a length of 399 bp was selected to be tested. We showed that the tobacco rattle virus (TRV)-VIGS could induce effective HbPDS silencing in rubber tree. This study was the first to report VIGS in rubber tree. The present TRV-VIGS method could be used to perform reverse genetic approaches to identify unknown gene functions and might be further applied to produce gene silenced rubber tree plants, to advance functional gene of rubber tree.

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