您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Genetic characterization of porcine sapoviruses identified from pigs during a diarrhoea outbreak in Iowa, 2019

文献类型： 外文期刊

作者： Shen, Huigang 1 ; Zhang, Jianfeng 1 ; Gauger, Phillip C. 1 ; Burrough, Eric R. 1 ; Zhang, Jianqiang 1 ; Harmon, Kar 1 ;

作者机构： 1.Iowa State Univ, Dept Vet Diagnost & Prod Anim Med, Coll Vet Med, Ames, IA 50011 USA

2.Guangdong Acad Agr Sci, Inst Anim Hlth, Key Lab Livestock Dis Prevent Guangdong Prov, Sci Observat & Expt Stn Vet Drugs & Diagnost Tech, Guangzhou, Peoples R China

3.Univ Illinois, Coll Vet Med, Dept Vet Clin Med, Urbana, IL 61801 USA

4.Univ Illinois, Coll Vet Med, Vet Diagnost Lab, Urbana, IL 61801 USA

5.Arkcare, Fairbury, NE USA

关键词： diarrhoea; phylogenetic analysis; porcine sapovirus; real‐ time PCR; recombination

期刊名称：TRANSBOUNDARY AND EMERGING DISEASES （ 影响因子：4.188； 五年影响因子：3.669 ）

ISSN： 1865-1674

年卷期：

页码：

收录情况： SCI

摘要： Porcine sapovirus (SaV) was first identified by electron microscopy in the United States in 1980 and has since been reported from both asymptomatic and diarrhoeic pigs usually in mixed infection with other enteric pathogens. SaV as the sole aetiological agent of diarrhoea in naturally infected pigs has not previously been reported in the United States. Here, we used four independent lines of evidence including metagenomics analysis, real-time RT-PCR (rRT-PCR), histopathology, and in situ hybridization to confirm porcine SaV genogroup III (GIII) as the sole cause of enteritis and diarrhoea in pigs. A highly sensitive and specific rRT-PCR was established to detect porcine SaV GIII. Examination of 184 faecal samples from an outbreak of diarrhoea on a pig farm showed that pigs with clinical diarrhoea had significantly lower C-t values (15.9 +/- 0.59) compared to clinically unaffected pigs (35.8 +/- 0.71). Further survey of 336 faecal samples from different states in the United States demonstrated that samples from pigs with clinical diarrhoea had a comparable positive rate (45.3%) with those from asymptomatic pigs (43.1%). However, the SaV-positive pigs with clinical diarrhoea had significantly higher viral loads (C-t = 26.0 +/- 0.5) than the SAV-positive but clinically healthy pigs (C-t = 33.2 +/- 0.9). Phylogenetic analysis of 20 field SaVs revealed that all belonged to SaV GIII and recombination analysis indicated that intragenogroup recombination had occurred within the field isolates of SaV GIII. These results suggest that porcine SaV GIII plays an important aetiologic role in swine enteritis and diarrhoea and rRT-PCR is a reliable method to detect porcine SaV. Our findings provide significant insights to better understand the epidemiology and pathogenicity of porcine SaV infection.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台