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Characterization of mandarin fish (Siniperca chuatsi) IL-6 and IL-6 signal transducer and the association between their SNPs and resistance to ISKNV disease

文献类型： 外文期刊

作者： Jin, Rui-Ming 1 ; Huang, He-Zhong 1 ; Zhou, Yu 1 ; Wang, Ying-Ying 1 ; Fu, Huang-Cui 1 ; Li, Ze 1 ; Fu, Xiao-Zhe 2 ; Li, 1 ;

作者机构： 1.Soochow Univ, Sch Basic Med & Biol Sci, Fisheries Res Inst, Suzhou 215123, Jiangsu, Peoples R China

2.Chinese Acad Fishery Sci, Pearl River Fisheries Res Inst, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs,Key Lab Aquat Anim Imm, Guangzhou 510380, Guangdong, Peoples R China

关键词： IL-6; IL-6ST; SNP; ISKNV-Resistant; Mandarin fish

期刊名称：FISH & SHELLFISH IMMUNOLOGY （ 影响因子：3.298； 五年影响因子：3.55 ）

ISSN： 1050-4648

年卷期： 2021 年 113 卷

页码：

收录情况： SCI

摘要： In fish, interleukin-6 (IL-6) is a very important immune-regulatory cytokine that plays a polyfunctional role in inflammation, metabolism, regeneration, and neural processes. IL-6 signal transducer (IL-6ST) is a specific receptor for IL-6 and expressed mainly in immune cells and hepatocytes. In this study, the complete cDNA and genomic DNA sequences of mandarin fish (Siniperca chuatsi) IL-6 and IL-6ST genes were identified and analyzed. Quantitative real-time PCR showed that IL-6 and IL-6ST were chiefly expressed in the immune organs. After challenge with infectious spleen and kidney necrosis virus (ISKNV), the expression levels of IL-6 were significantly up-regulated after 6 h and 24 h in the head kidney and spleen, respectively (p < 0.01), the peak value for both reached at 72 h, IL-6ST increased significantly after 120 h with a peak at 168 h in the head kidney (p < 0.01) and improved markedly at 168 h in the spleen (p < 0.01). Besides, IL-6 and IL-6ST have been identified 3 and 8 single nucleotide polymorphisms (SNPs), respectively. Statistical analysis showed that one SNP locus (1625C/T) in the coding region of IL-6 was significantly related to the resistance of mandarin fish against ISKNV. The 1625C -> T locus in the coding region of IL-6 is a synonymous mutation; compared with the susceptible group, the frequency of allele T in the disease resistance group was significantly higher, which may be due to the rare codon produced by the mutation affecting translation. The involvement of IL-6 and IL-6ST in response to ISKNV infection in mandarin fish clearly indicate that the role of SNP markers in IL-6 was associated with the ISKNV resistance, which was demonstrated for the first time in our results. Thus, the current study may provide fundamental information for further breeding of mandarin fish with resistance to ISKNV infection.

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