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A teleost bactericidal permeability-increasing protein-derived peptide that possesses a broad antibacterial spectrum and inhibits bacterial infection as well as human colon cancer cells growth

文献类型： 外文期刊

作者： Gu, Qin-Qin 1 ; He, Shu-Wen 1 ; Liu, Li-Hui 2 ; Wang, Guang-Hua 1 ; Hao, Dong-Fang 1 ; Liu, Hong-Mei 1 ; Wang, Chang-Biao 1 ; Li, Chao 1 ; Zhang, Min 1 ; Li, Ning-Qiu 2 ;

作者机构： 1.Qingdao Agr Univ, Sch Marine Sci & Engn, Qingdao 266109, Shandong, Peoples R China

2.Chinese Acad Fishery Sci, Key Lab Fishery Drug Dev, Minist Agr & Rural Affairs, Key Lab Aquat Anim Immune Technol,Pearl River Fis, Guangzhou 510380, Guangdong, Peoples R China

3.Pilot Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Shandong, Peoples R China

关键词： Antibacterial peptide; BO18; Antibacterial mechanism; Immunoregulatory activity; Cytotoxic effect

期刊名称：DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY （ 影响因子：3.605； 五年影响因子：3.833 ）

ISSN： 0145-305X

年卷期： 2021 年 118 卷

页码：

收录情况： SCI

摘要： The bactericidal permeability-increasing protein (BPI) is a multifunctional cationic protein produced by neutrophils with antibacterial, antitumor, and LPS-neutralizing properties. In teleost, a number of BPIs have been reported, but their functions are very limited. In this study, an N-terminal peptide, BO18 (with 18 amino acids), derived from rock bream (Oplegnathus fasciatus) BPI, was synthesized and investigated for its antibacterial spectrum, action mechanism, immunoregulatory property as well as the inhibition effects on bacterial invasion and human colon cancer cells growth. The results showed that BO18 was active against Gram-positive bacteria Bscillus subiilis, Micrococcus luteus, and Staphylococcus aureus, as well as Gram-negative bacteria Vibrio alginolyticus, Vibrio litoralis, Vibrio parahaemolyticus and Vibrio vulnificus. BO18 treatment facilitated the bactericidal process of erythromycin and rifampicin by enhancing the permeability of the outer membrane. During its interaction with V. alginolyticus, BO18 exerted its antibacterial activity by destroying cell membrane integrity, penetrating into the cytoplasm and binding to genomic DNA and total RNA. In vitro analysis indicated BO18 could enhance the respiratory burst ability and regulate the expression of immune related genes of macrophages. In vivo detection showed the administration of fish with BO18 before bacterial infection significantly reduced pathogen dissemination and replication in tissues. In addition, BO18 exerted a cytotoxic effect on the growth of human colon cancer cells HT-29. Together, these results add new insights into the function of teleost BPIs, and support that BO18 is a novel and broad-spectrum antibacterial peptide with potential to apply in fighting pathogenic infection in aquaculture.

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