TGF-β1 impairs IgA class switch recombination and production in porcine Peyer's patches B cells
文献类型： 外文期刊
作者： Wang, Caiying 1 ; Zhang, Yue 1 ; Lu, Yabin 4 ; Huang, Xin 1 ; Jiang, Huazheng 1 ; Chen, Guohui 1 ; Shao, Yongheng 1 ; Savelkoul, Huub F. J. 3 ; Jansen, Christine A. 3 ; Liu, Guangliang 1 ;
作者机构： 1.Lanzhou Univ, State Key Lab Anim Dis Control & Prevent, Coll Vet Med, Lanzhou, Peoples R China
2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, Lanzhou, Peoples R China
3.Wageningen Univ & Res, Cell Biol & Immunol Grp, Wageningen, Netherlands
4.Xinjiang Agr Univ, Coll Vet Med, Urumqi, Peoples R China
5.Hainan Acad Agr Sci, Inst Anim Husb & Vet Med, Hainan Key Lab Trop Anim Breeding & Infect Dis Re, Haikou, Hainan, Peoples R China
6.Gansu Prov Res Ctr Basic Disciplines Pathogen Bio, Lanzhou, Peoples R China
关键词： IgA; IgM(+) B cells; p38-MAPK; Peyer's patches; TGF-beta 1
期刊名称：EUROPEAN JOURNAL OF IMMUNOLOGY （ 影响因子：4.5； 五年影响因子：4.6 ）
ISSN： 0014-2980
年卷期： 2024 年
页码：
收录情况： SCI
摘要： Secretory IgA is crucial for preventing the invasion of entero-pathogens via intestinal mucosa. While it is well-established that Transforming growth factor beta 1 (TGF-beta 1) regulates IgA production in human and mouse B cells, our previous investigation revealed different functions of TGF-beta 1 in IgA generation in pigs compared with humans and mice, with the underlying mechanism remaining elusive. In this study, IgM+ B cells from porcine Peyer's patches (PPs) were isolated and stimulated with recombinant porcine TGF-beta 1 to evaluate the effect of TGF-beta 1 on pigs. The results showed that antibody production from B cells of PPs was impaired by TGF-beta 1 ex vivo. Furthermore, TGF-beta 1 treatment led to a decrease in the expression of germ-line transcript alpha and postswitch transcript alpha. Moreover, we observed that TGF-beta 1 predominantly inhibited the phosphorylation of p38-mitogen-activated protein kinases (MAPK), confirming the involvement of the p38-MAPK pathway in porcine IgA generation and IgA class switch recombination. The application of p38-MAPK inhibitor resulted in decreased B-cell differentiation levels. Collectively, this study demonstrates that exogenous TGF-beta 1 restrains the production and class switch recombination of IgA antibodies by inhibiting p38-MAPK signaling in porcine PPs B cells, which may constitute a component of TGF-beta 1-mediated inhibition of B-cell activation.
- 相关文献
作者其他论文 更多>>
-
First determination of diagnostic doses of the pesticide-sensitive strain of Haemaphysalis longicornis in China
作者：Diao, Peiwen;Luo, Jin;Ren, Qiaoyun;Wang, Jinming;Cao, Runlai;Guo, Junhui;Guan, Guiquan;Luo, Jianxun;Yin, Hong;Liu, Guangliang;Liu, Guangyuan;Ren, Qiaoyun;Zhang, Yan;Luo, Jianxun;Yin, Hong
关键词：ticks; pesticide-sensitive strain; Haemaphysalis longicornis; discriminating concentration
-
Potassium molybdate blocks APN-dependent coronavirus entry by degrading receptor via PIK3C3-mediated autophagy
作者：Zhang, Yunhang;Zhang, Yue;Li, Yang;Yang, Ning;Cai, Yifei;Tan, Chen;Zhao, Jing;Li, Wenjie;Liu, Yuanyuan;Rui, Xue;Wu, Junfei;Fu, Yuguang;Liu, Guangliang;Zhang, Yunhang;Yang, Ning;Tan, Chen;Zhang, Yunhang;Yang, Ning;Tan, Chen;Zhang, Yunhang;Zhang, Na;Yang, Ning;Cai, Yifei;Tan, Chen;Liu, Yuanyuan;Rui, Xue;Liu, Guangliang;Cai, Yifei;Liu, Yuanyuan;Rui, Xue;Liu, Guangliang
关键词：potassium molybdate; APN-dependent coronavirus; autophagy
-
In vitro and in vivo evaluation of thapsigargin as an antiviral agent against transmissible gastroenteritis virus
作者：Li, Yang;Liu, Yuanyuan;Zhang, Yunhang;Tan, Chen;Cai, Yifei;Zhang, Yue;Chen, Jianing;Fu, Yuguang;Liu, Guangliang;Liu, Yuanyuan;Liu, Guangliang;Liu, Yuanyuan;Zhang, Yunhang;Tan, Chen;Cai, Yifei
关键词：thapsigargin; intestinal organoids; ERS; antiviral drug; TGEV
-
Unveiling the Influence of Copy Number Variations on Genetic Diversity and Adaptive Evolution in China's Native Pig Breeds via Whole-Genome Resequencing
作者：Yuan, Haonan;Wei, Wenjing;Zhang, Yue;Zhao, Shengguo;Quan, Jinqiang;Li, Changwen;Xia, Changyou;Gao, Caixia;Chao, Zhe
关键词：genomic analysis; selective breeding; gene-environment interaction; swine genomics; animal model applications
-
Hypoxia inducible factor-1α facilitates transmissible gastroenteritis virus replication by inhibiting type I and type III interferon production
作者：Zhang, Yunhang;Rui, Xue;Li, Yang;Zhang, Yue;Cai, Yifei;Tan, Chen;Yang, Ning;Liu, Yuanyuan;Fu, Yuguang;Liu, Guangliang;Zhang, Yunhang;Tan, Chen;Yang, Ning;Zhang, Yunhang;Rui, Xue;Cai, Yifei;Tan, Chen;Yang, Ning;Liu, Yuanyuan;Liu, Guangliang;Cai, Yifei;Rui, Xue;Liu, Yuanyuan;Liu, Guangliang;Liu, Guangliang
关键词：TGEV; HIF-1 alpha; Intestinal organoid monolayer; Type I and type III IFN production; In vivo
-
Wuzhishan miniature pig-derived intestinal 2D monolayer organoids to investigate the enteric coronavirus infection
作者：Liu, Yuanyuan;Tan, Chen;Zhang, Yunhang;Gao, Shuai;Cai, Yifei;Liu, Guangliang;Li, Yang;Liu, Yuanyuan;Yang, Ning;Tan, Chen;Zhang, Yunhang;Cai, Yifei;Zhang, Yue;Fu, Yuguang;Liu, Guangliang;Li, Yang;Liu, Yuanyuan;Yang, Ning;Tan, Chen;Zhang, Yunhang;Cai, Yifei
关键词：intestinal organoids; organoids monolayers; RNA-seq; TGEV; Wuzhishan pigs
-
A mucus layer derived from porcine intestinal organoid air-liquid interface monolayer attenuates swine enteric coronavirus infection by antiviral activity of Muc2
作者：Yang, Ning;Li, Yang;Cai, Yifei;Liu, Yuanyuan;Zhang, Yunhang;Fu, Yuguang;Tan, Chen;Liu, Guangliang;Yang, Ning;Zhang, Yunhang;Tan, Chen;Willems, Luc;Yang, Ning;Li, Yang;Cai, Yifei;Liu, Yuanyuan;Zhang, Yunhang;Tan, Chen;Liu, Guangliang;Cai, Yifei;Liu, Yuanyuan;Liu, Guangliang
关键词：Coronavirus; Intestinal organoid monolayer; Air-liquid interface (ALI); Mucus layer; Muc2; Sialic acid
