Wuzhishan miniature pig-derived intestinal 2D monolayer organoids to investigate the enteric coronavirus infection
文献类型： 外文期刊
作者： Liu, Yuanyuan 1 ; Yang, Ning 2 ; Tan, Chen 1 ; Zhang, Yunhang 1 ; Gao, Shuai 1 ; Cai, Yifei 1 ; Zhang, Yue 2 ; Fu, Yuguang 2 ; Liu, Guangliang 1 ; Li, Yang 1 ;
作者机构： 1.Hainan Acad Agr Sci, Inst Anim Husb & Vet Med, Key Lab Trop Anim Breeding & Dis Res, Haikou, Peoples R China
2.Chinese Acad Agr Sci, Lanzhou Vet Res Inst, State Key Lab Anim Dis Control & Prevent, Lanzhou, Peoples R China
3.Xinjiang Agr Univ, Coll Vet Med, Urumqi, Peoples R China
4.Univ Liege, Mol & Cellular Epigenet GIGA, Liege, Belgium
5.Wageningen Univ & Res, Human Nutr & Hlth Grp, VLAG, Wageningen, Netherlands
关键词： intestinal organoids; organoids monolayers; RNA-seq; TGEV; Wuzhishan pigs
期刊名称：FRONTIERS IN VETERINARY SCIENCE （ 影响因子：2.9； 五年影响因子：3.3 ）
ISSN：
年卷期： 2024 年 11 卷
页码：
收录情况： SCI
摘要： Intestinal organoids are valuable tools for investigating intestinal physiology and pathology ex vivo. In previous studies, intestinal organoids of commercial pigs have been developed. Here, we established intestinal organoids derived from Wuzhishan miniature pigs (WZS pigs), a unique kind of pig in the Hainan province of China. Three-dimensional (3D) intestinal organoids and organoid monolayers were developed and assessed. Furthermore, the susceptibility of organoid monolayers of WZS pigs to transmissible gastroenteritis virus (TGEV) was demonstrated. An RNA-seq analysis revealed that the TGEV infection stimulated antiviral and inflammatory immune responses in organoid monolayer models. The study implied the transmission risk of swine enteric coronavirus on WZS pigs and provided useful tools for further research on WZS pigs as laboratory miniature pig models.
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