Characterization of a Galactose Oxidase from Fusarium odoratissimum and Its Application in the Modification of Agarose
文献类型： 外文期刊
作者： Cao, Na 1 ; Xia, Guangli 1 ; Sun, Huihui 2 ; Zhao, Ling 2 ; Cao, Rong 2 ; Jiang, Hong 1 ; Mao, Xiangzhao 1 ; Liu, Qi 2 ;
作者机构： 1.Ocean Univ China, Coll Food Sci & Engn, Qingdao 266003, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Dept Food Engn & Nutr, Qingdao 266071, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Drugs & Bioprod, Qingdao 266237, Peoples R China
关键词： galactose oxidase; Fusarium odoratissimum; enzyme characterization; oxidation modification; polyaldehyde-based polymer; agarose
期刊名称：FOODS （ 影响因子：5.2； 五年影响因子：5.5 ）
ISSN：
年卷期： 2023 年 12 卷 3 期
页码：
收录情况： SCI
摘要： A galactose oxidase gene, gao-5f, was cloned from Fusarium odoratissimum and successfully expressed in E. coli. The galactose oxidase GAO-5F belongs to the AA5 family and consists of 681 amino acids, with an estimated molecular weight of 72 kDa. GAO-5F exhibited maximum activity at 40 degrees C and pH 7.0 and showed no change in activity after 24 h incubation at 30 degrees C. Moreover, GAO-5F exhibited 40% of its maximum activity after 24 h incubation at 50 degrees C and 60% after 40 h incubation at pH 7.0. The measured thermostability of GAO-5F is superior to galactose oxidase's reported thermostability. The enzyme exhibited strict substrate specificity toward D-galactose and oligosaccharides/polysaccharides containing D-galactose. Further analysis demonstrated that GAO-5F specifically oxidized agarose to a polyaldehyde-based polymer, which could be used as a polyaldehyde to crosslink with gelatin to form edible packaging films. To our knowledge, this is the first report about the modification of agarose by galactose oxidase, and this result has laid a foundation for the further development of edible membranes using agarose.
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