您好，欢迎访问新疆农业科学院 机构知识库！
新疆农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Cell-specific clock-controlled gene expression program regulates rhythmic fiber cell growth in cotton

文献类型： 外文期刊

作者： Wang, Dehe 1 ; Hu, Xiao 1 ; Ye, Hanzhe 1 ; Wang, Yue 1 ; Yang, Qian 1 ; Liang, Xiaodong 1 ; Wang, Zilin 1 ; Zhou, Yifan 1 ; Wen, Miaomiao 3 ; Yuan, Xueyan 1 ; Zheng, Xiaomin 1 ; Ye, Wen 5 ; Guo, Boyu 1 ; Yusuyin, Mayila 8 ; Russinova, Eugenia 6 ; Zhou, Yu 1 ; Wang, Kun 1 ;

作者机构： 1.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan, Peoples R China

2.Hubei Hongshan Lab, Wuhan, Peoples R China

3.Wuhan Univ, Inst Adv Studies, Wuhan, Peoples R China

4.Wuhan Univ, Remin Hosp, RNA Inst, TaiKang Ctr Life & Med Sci, Wuhan, Peoples R China

5.Wuhan Univ, Med Res Inst, Frontier Sci Ctr Immunol & Metab, Sch Med, Wuhan, Peoples R China

6.Univ Ghent, Dept Plant Biotechnol & Bioinformat, Ghent, Belgium

7.Ctr Plant Syst Biol VIB, Ghent, Belgium

8.Xinjiang Acad Agr Sci, Res Inst Econ Crops, Urumqi, Peoples R China

关键词： scRNA-seq; Rhythmic regulation; Fiber development; Cotton

期刊名称：GENOME BIOLOGY （ 影响因子：12.3； 五年影响因子：17.4 ）

ISSN： 1474-760X

年卷期： 2023 年 24 卷 1 期

页码：

收录情况： SCI

摘要： BackgroundThe epidermis of cotton ovule produces fibers, the most important natural cellulose source for the global textile industry. However, the molecular mechanism of fiber cell growth is still poorly understood.ResultsHere, we develop an optimized protoplasting method, and integrate single-cell RNA sequencing (scRNA-seq) and single-cell ATAC sequencing (scATAC-seq) to systematically characterize the cells of the outer integument of ovules from wild type and fuzzless/lintless (fl) cotton (Gossypium hirsutum). By jointly analyzing the scRNA-seq data from wildtype and fl, we identify five cell populations including the fiber cell type and construct the development trajectory for fiber lineage cells. Interestingly, by time-course diurnal transcriptomic analysis, we demonstrate that the primary growth of fiber cells is a highly regulated circadian rhythmic process. Moreover, we identify a small peptide GhRALF1 that circadian rhythmically controls fiber growth possibly through oscillating auxin signaling and proton pump activity in the plasma membrane. Combining with scATAC-seq, we further identify two cardinal cis-regulatory elements (CREs, TCP motif, and TCP-like motif) which are bound by the trans factors GhTCP14s to modulate the circadian rhythmic metabolism of mitochondria and protein translation through regulating approximately one third of genes that are highly expressed in fiber cells.ConclusionsWe uncover a fiber-specific circadian clock-controlled gene expression program in regulating fiber growth. This study unprecedentedly reveals a new route to improve fiber traits by engineering the circadian clock of fiber cells.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：新疆农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台