文献类型： 外文期刊

作者： Wang, Hongjie ¹ ; Li, Cheng ¹ ; Wang, Lidan ¹ ; Zhong, Hongying ¹ ; Xu, Xin ¹ ; Cheng, Yanbo ¹ ; Nian, Hai ¹ ; Liu, Wenhua ⁶ ; Chen, Pei ⁶ ; Zhang, Aixia ⁶ ; Ma, Qibin ¹ ;

作者机构： 1.South China Agr Univ, State Key Lab Conservat & Utilizat Subtrop Agrobio, Guangzhou, Peoples R China

2.South China Agr Univ, Coll Agr, Guangdong Prov Key Lab Plant Mol Breeding, Guangzhou, Peoples R China

3.South China Agr Univ, Coll Agr, Guangdong Subctr Natl Ctr Soybean Improvement, Guangzhou, Peoples R China

4.South China Agr Univ, Guangdong Prov Lab Lingnan Modern Agr Sci & Techno, Guangzhou, Peoples R China

5.South China Agr Univ, Zengcheng Teaching & Res Bases, Guangzhou, Guangdong, Peoples R China

6.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangzhou, Guangdong, Peoples R China

关键词： GmABR1; soybean; Al stress; transgenic Arabidopsis thaliana; transcription factor

期刊名称：FRONTIERS IN PLANT SCIENCE （ 影响因子：5.6； 五年影响因子：6.8 ）

ISSN： 1664-462X

年卷期： 2023 年 14 卷

页码：

收录情况： SCI

摘要： The ethylene response factor (ERF) transcription factors, which is one of the largest transcription factor families in plants, are involved in biological and abiotic stress response and play an important role in plant growth and development. In this study, the GmABR1 gene from the soybean inbred line Zhonghuang24 (ZH24)xHuaxia 3 (HX3) was investigated its aluminum (Al) tolerance. GmABR1 protein has a conserved domain AP2, which is located in the nucleus and has transcriptional activation ability. The results of real-time quantitative PCR (qRT-PCR) showed that the GmABR1 gene presented a constitutive expression pattern rich in the root tip, stem and leaf tissues of HX3. After Al stress, the GmABR1 transcript was significantly increased in the roots. The transcripts of GmABR1 in the roots of HX3 treated with 50 mu M AlCl3 was 51 times than that of the control. The GmABR1 was spatiotemporally specific with the highest expression levels when Al concentration was 50 mu M, which was about 36 times than that of the control. The results of hematoxylin staining showed that the root tips of GmABR1-overexpression lines were stained the lightest, followed by the control, and the root tips of GmABR1 RNAi lines were stained the darkest. The concentrations of Al3+ in root tips were 207.40 mu g/g, 147.74 mu g/g and 330.65 mu g/g in wild type (WT), overexpressed lines and RNAi lines, respectively. When AlCl3 (pH4.5) concentration was 100 mu M, all the roots of Arabidopsis were significantly inhibited. The taproot elongation of WT, GmABR1 transgenic lines was 69.6%, 85.6%, respectively. When treated with Al, the content of malondialdehyde (MDA) in leaves of WT increased to 3.03 mu g/g, while that of transgenic Arabidopsis increased from 1.66-2.21 mu g/g, which was lower than that of WT. Under the Al stress, the Al stress responsive genes such as AtALMT1 and AtMATE, and the genes related to ABA pathway such as AtABI1, AtRD22 and AtRD29A were up-regulated. The results indicated that GmABR1 may jointly regulate plant resistance to Al stress through genes related to Al stress response and ABA response pathways.