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OsNAC5 orchestrates OsABI5 to fine-tune cold tolerance in rice

文献类型： 外文期刊

作者： Li, Ruiqing 1 ; Song, Yue 2 ; Wang, Xueqiang 2 ; Zheng, Chenfan 2 ; Liu, Bo 2 ; Zhang, Huali 4 ; Ke, Jian 1 ; Wu, Xuejing 1 ; Wu, Liquan 1 ; Yang, Ruifang 6 ; Jiang, Meng 2 ;

作者机构： 1.Anhui Agr Univ, Coll Agron, Hefei 230036, Peoples R China

2.Zhejiang Univ, Hainan Inst, Yazhou Bay Sci Tech City, Sanya 572025, Peoples R China

3.Zhejiang Univ, Adv Seed Inst, Natl Key Lab Rice Biol, Hangzhou 311225, Peoples R China

4.China Natl Rice Res Inst, State Key Lab Rice Biol, Hangzhou 311401, Peoples R China

5.China Natl Rice Res Inst, Chinese Natl Ctr Rice Improvement, Hangzhou 311401, Peoples R China

6.Shanghai Acad Agr Sci, Key Lab Germplasm Innovat & Genet Improvement Grai, Minist Agr & Rural Affairs, Crop Breeding & Cultivat Res Inst, Shanghai 201106, Peoples R China

关键词： ABA signaling; ABI5; cold tolerance; DREB; NAC5; rice

期刊名称：JOURNAL OF INTEGRATIVE PLANT BIOLOGY （ 影响因子：11.4； 五年影响因子：10.1 ）

ISSN： 1672-9072

年卷期： 2024 年 66 卷 4 期

页码：

收录情况： SCI

摘要： Due to its tropical origins, rice (Oryza sativa) is susceptible to cold stress, which poses severe threats to production. OsNAC5, a NAC-type transcription factor, participates in the cold stress response of rice, but the detailed mechanisms remain poorly understood. Here, we demonstrate that OsNAC5 positively regulates cold tolerance at germination and in seedlings by directly activating the expression of ABSCISIC ACID INSENSITIVE 5 (OsABI5). Haplotype analysis indicated that single nucleotide polymorphisms in a NAC-binding site in the OsABI5 promoter are strongly associated with cold tolerance. OsNAC5 also enhanced OsABI5 stability, thus regulating the expression of cold-responsive (COR) genes, enabling fine-tuned control of OsABI5 action for rapid, precise plant responses to cold stress. DNA affinity purification sequencing coupled with transcriptome deep sequencing identified several OsABI5 target genes involved in COR expression, including DEHYDRATION-RESPONSIVE ELEMENT BINDING FACTOR 1A (OsDREB1A), OsMYB20, and PEROXIDASE 70 (OsPRX70). In vivo and in vitro analyses suggested that OsABI5 positively regulates COR gene transcription, with marked COR upregulation in OsNAC5-overexpressing lines and downregulation in osnac5 and/or osabi5 knockout mutants. This study extends our understanding of cold tolerance regulation via OsNAC5 through the OsABI5-CORs transcription module, which may be used to ameliorate cold tolerance in rice via advanced breeding.

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