Fermentation of NaHCO3-treated corn germ meal by Bacillus velezensis CL-4 promotes lignocellulose degradation and nutrient utilization
文献类型： 外文期刊
作者： Chen, Long 1 ; Chen, Wanying 1 ; Zheng, Boyu 1 ; Yu, Wei 1 ; Zheng, Lin 1 ; Qu, Zihui 1 ; Yan, Xiaogang 1 ; Wei, Bingdong 1 ; Zhao, Zijian 2 ;
作者机构： 1.Jilin Acad Agr Sci, Inst Anim Nutr & Feed, 186 Dong Xinghua St, Gongzhuling 136100, Jilin, Peoples R China
2.Jilin Acad Agr Sci, Inst Agrofood Technol, 1366 Cai Yu St, Changchun 130033, Jilin, Peoples R China
关键词： Bacillus velezensis CL-4; Corn germ meal; Lignocellulose-degrading enzyme; Sodium bicarbonate; Fermented feed
期刊名称：APPLIED MICROBIOLOGY AND BIOTECHNOLOGY （ 影响因子：5.56； 五年影响因子：5.365 ）
ISSN： 0175-7598
年卷期： 2022 年 106 卷 18 期
页码：
收录情况： SCI
摘要： Sodium bicarbonate pretreatment and solid-state fermentation (SSF) were used to maximize the nutritional value of corn germ meal (CGM) by inoculating it with Bacillus velezensis CL-4 (isolated from chicken cecal contents and capable of degrading lignocellulose). Based on genome sequencing, B. velezensis CL-4 has a 4,063,558 bp ring chromosome and 46.27% GC content. Furthermore, genes associated with degradation of lignocellulose degradation were detected. Pretreatment of CGM (PCGM) with sodium bicarbonate (optimized to 0.06 g/mL) neutralized low pH. Fermented and pretreated CGM (FPCGM) contained more crude protein (CP), soluble protein of trichloroacetic acid (TCA-SP), and total amino acids (aa) than CGM and PCGM. Degradation rates of cellulose and hemicellulose were reduced by 21.33 and 71.35%, respectively, after 48 h fermentation. Based on electron microscopy, FPCGM destroys the surface structure and adds small debris of the CGM substrate, due to lignocellulose breakdown. Furthermore, 2-oxoadipic acid and dimethyl sulfone were the most important metabolites during pretreatment. Concentrations of adenosine, cytidine, guanosine, S-methyl-5'-thioadenosine, and adenine decreased significantly after 48 h fermentation, whereas concentrations of probiotics, enzymes, and fatty acids (including palmitic, 16-hydroxypalmitic, and linoleic acids) were significantly improved after fermentation. In conclusion, the novel pretreatment of CGM provided a proof of concept for using B. velezensis CL-4 to degrade lignocellulose components, improve nutritional characteristics of CGM, and expand CGM lignocellulosic biological feed production.
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