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Multiplex TaqMan real-time PCR assay for high-throughput identification of highly toxic mushroom species-induced foodborne poisoning

文献类型： 外文期刊

作者： Zhao, Lanxin 1 ; Zhao, Xiaoyan 1 ; Hengchao, E. 1 ; Fan, Tingting 1 ; Tian, Enjing 3 ; Wang, Chunling 4 ; Zhang, Yanmei 1 ; Li, Xiaobei 1 ; Zhao, Zhiyong 1 ; Zhou, Changyan 1 ;

作者机构： 1.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, Lab Qual & Safety Risk Assessment Agroprod Shangha, Minist Agr & Rural Affairs, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

2.Shanghai Ocean Univ, Coll Food Sci & Technol, Shanghai 201306, Peoples R China

3.Jilin Agr Univ, Engn Res Ctr, Chinese Minist Educ Edible & Med Fungi, Changchun 130118, Jilin, Peoples R China

4.Fudan Univ, Minhang Hosp, Inst Fudan Minhang Acad Hlth Syst, Shanghai 201100, Peoples R China

5.Shanghai Guosen Biotechnol Co Ltd, Shanghai 201400, Peoples R China

6.Shanghai Acad Agr Sci, Inst Agrofood Stand & Testing Technol, 1000 Jinqi Rd, Shanghai 201403, Peoples R China

关键词： Highly toxic mushroom species; Multiplex real-time PCR; Simultaneous identification; Foodborne poisoning

期刊名称：LWT-FOOD SCIENCE AND TECHNOLOGY （ 影响因子：6.0； 五年影响因子：6.0 ）

ISSN： 0023-6438

年卷期： 2023 年 186 卷

页码：

收录情况： SCI

摘要： The highly toxic Amanita species, Russula subnigricans, and Lepiota brunneoincarnata are the major mushroom types causing mushroom poisoning fatalities worldwide, which seriously endanger human life and health. However, the detection method for the simultaneous identification of these highly toxic mushroom types is scarce. In this study, a one-tube multiplex TaqMan real-time PCR assay was established for simultaneous detection of the highly toxic mushroom species from three genera. The developed assay could simultaneously detect at least 6 highly toxic Amanita species and specially identify R. subnigricans and L. brunneoincarnata without the cross-reaction in 37 mushroom species. The method detection limits were 0.1 pg/& mu;L, 1 pg/& mu;L, and 0.1 pg/& mu;L DNA for highly toxic Amanita species, R. subnigricans, and L. brunneoincarnata with high amplification efficiency. Moreover, the multiplex real-time PCR assay was successfully applied to the cooked mushroom. The developed method is expected to become an effective tool for high-throughput identification of foodborne poisoning associated with these highly toxic mushroom species.

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