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Effect of HDAC9 on the differentiation of chicken embryonic stem cells into male germ cells

文献类型： 外文期刊

作者： Li, Xin 1 ; Yu, Yongsheng 1 ; Zhang, Qi 1 ; Luo, Xiaotong 1 ; Yu, Li 2 ; Zhao, Zhongli 1 ;

作者机构： 1.Jilin Acad Agr Sci, Inst Anim Husb & Vet Med, Jilin, Peoples R China

2.Anim Husb Stn, Jilin, Peoples R China

关键词： cESCs; HDAC9; male germ cells; gene regulation; TMP195.

期刊名称：ANIMAL REPRODUCTION （ 影响因子：1.6； 五年影响因子：2.1 ）

ISSN： 1806-9614

年卷期： 2024 年 21 卷 2 期

页码：

收录情况： SCI

摘要： Histone deacetylase 9 (HDAC9) is a histone deacetylase (HDAC) subtype IIa protein that deacetylates histone 3 (H3), histone 4 (H4), and nonhistone proteins in vivo to alter chromosomal shape and regulate gene transcription. There have been few studies on the regulatory influence of the HDAC9 gene on the differentiation of chicken embryonic stem cells (cESCs) into male germ cells, and the significance of HDAC9 is still unknown. Therefore, we explored the specific role of HDAC9 during differentiation of the cESCs of Jilin Luhua chickens through inhibition or overexpression. In medium supplemented with 10-5 mol/L retinoic acid (RA), cESCs were stimulated to develop into germ cells. HDAC9 and germline marker gene mRNA and protein levels were measured using qRT-PCR and western blotting. During the differentiation of cESCs into male germ cells, overexpression of the HDAC9 gene greatly increased the mRNA and protein expression levels of the germline marker genes Stra8, Dazl, c-kit, and integrin alpha 6. The HDAC9 inhibitor TMP195 significantly decreased the mRNA and protein expression levels of the above markers. In summary, HDAC9 positively regulates the differentiation of cESCs.

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