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Follicular fluid exosome-derived miR-339-5p enhances in vitro maturation of porcine oocytes via targeting SFPQ , a regulator of the ERK1/2 pathway

文献类型： 外文期刊

作者： Han, Yue 1 ; Zhang, Junzheng 1 ; Liang, Wanfeng 1 ; Lv, Yanqiu 1 ; Luo, Xiaotong 2 ; Li, Chunyu 1 ; Qu, Xinglin 1 ; Zhang, Yuyang 1 ; Gu, Weiyu 1 ; Chen, Xuan 1 ; Jin, Yi 1 ;

作者机构： 1.Yanbian Univ, Yanji 133000, Jilin, Peoples R China

2.Jilin Acad Agr Sci, Inst Anim Biotechnol, Gongzhuling 136100, Jilin, Peoples R China

关键词： Follicular fluid exosomes; miR-339-5p; Oocyte quality; SFPQ; ERK1/2

期刊名称：THERIOGENOLOGY （ 影响因子：2.4； 五年影响因子：2.5 ）

ISSN： 0093-691X

年卷期： 2024 年 225 卷

页码：

收录情况： SCI

摘要： In this study, we aimed to investigate cytoplasmic maturation and miRNA expression of mature oocytes cultured in porcine follicular fluid exosomes. We also examined the effect of miR-339-5p on oocyte maturation. Twenty eight differentially expressed miRNAs were detected using miRNA-seq. We then transfected cumulus oocyte complexes with miR-339-5p mimics and inhibitor during culture. The results showed that exosomes increased endoplasmic reticulum levels and the amount of lipid droplets, and decreased ROS levels, lipid droplet size, and percentage of oocytes with abnormal cortical granule distribution. Overexpressing miR-339-5p significantly decreased cumulus expansion genes, oocyte maturation-related genes, target gene proline/glutamine-rich splicing factor ( SFPQ ), ERK1/2 phosphorylation levels, oocyte maturation rate, blastocyst rate, and lipid droplet number, but increased lipid droplet size and the ratio of oocytes with abnormal cortical granule distribution. Inhibiting miR-339-5p reversed the decrease observed during overexpression. Mitochondrial membrane potential and ROS levels did not differ significantly between groups. In summary, exosomes promote oocyte cytoplasmic maturation and miR-339-5p regulating ERK1/2 activity through SFPQ expression, thereby elevating oocyte maturation and blastocyst formation rate in vitro.

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