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Effects of puerarin on the Akt signaling pathway in bovine preadipocyte differentiation

文献类型： 外文期刊

作者： Yun, Jinyan 1 ; Yu, Yongsheng 1 ; Zhou, Guoli 3 ; Luo, Xiaotong 1 ; Jin, Haiguo 1 ; Zhao, Yumin 1 ; Cao, Yang 1 ;

作者机构： 1.Jilin Acad Agr Sci, Branch Anim Husb, Changchun 130033, Jilin, Peoples R China

2.Minist Agr & Rural Agr, Key Lab Beef Cattle Genet & Breeding, Changchun 130033, Jilin, Peoples R China

3.Liaocheng Univ, Coll Life Sci, Liaocheng 252000, Shandong, Peoples R China

关键词： Puerarin; Adipogenesis; Akt Signaling Pathway; Preadipocyte Differentiation; Bovine

期刊名称：ASIAN-AUSTRALASIAN JOURNAL OF ANIMAL SCIENCES （ 影响因子：2.509； 五年影响因子：2.604 ）

ISSN： 1011-2367

年卷期： 2020 年 33 卷 1 期

页码：

收录情况： SCI

摘要： Objective: Puerarin has the potential of regulating the differentiation of preadipocytes, but its mechanism of action has not yet been elucidated. Adipocytes found in adipose tissue, the main endocrine organ, are the main sites of lipid deposition, and are widely used as a cell model in the study of in vitro fat deposition. This study aimed to investigate the effects of puerarin on adipogenesis in vitro. Methods: Puerarin was added to the culture medium during the process of adipogenesis. The proliferation and differentiation of bovine preadipocytes was measured through cell viability and staining with oil red O. The content of triacylglycerol was measured using a triglyceride assay kit. The mRNA and protein expression levels of adipogenic genes, peroxisome proliferator-activated receptor-gamma (PPAR gamma) and CCAAT/enhancer-binding protein-a, were measured using quantitative real-time polymerase chain reaction and western blotting, respectively. Results: The addition of puerarin significantly increased adipogenesis of bovine preadipocytes and enhanced the mRNA and protein level expression of PPAR gamma (p<0.01). The expression of P-Akt increased after adipogenic hormonal induction, whereas puerarin significantly increased PPAR gamma expression by promoting the Akt signaling component, P-Akt. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473, which may activate the downstream signaling of the Akt pathway. Conclusion: Puerarin was able to promote the differentiation of preadipocytes and improve fat deposition in cattle. The mechanism of adipogenesis was found to be related to the phosphorylation level of Ser473.

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