De novo genome assembly of Yanbian cattle using PacBio HiFi and Hi-C combined with RNA-seq data
文献类型: 外文期刊
作者: Fang, Wenwen 1 ; Cao, Yang 1 ; Liu, Yu 1 ; Wu, Jian 1 ; Miao, Lisheng 1 ; Xiao, Cheng 1 ; Li, Xin 1 ; Lv, Aihui 3 ; Zhao, Yumin 1 ; Cao, Yang 1 ;
作者机构: 1.Jilin Acad Agr Sci, Inst Anim Husb & Vet Med, Northeast Agr Res Ctr China, Gongzhuling 136100, Peoples R China
2.Minist Agr & Rural Affairs, Key Lab Beef Cattle Genet & Breeding, Gongzhuling 136100, Jilin, Peoples R China
3.Yanbian Livestock Dev Corp, Yanji 133000, Jilin, Peoples R China
期刊名称:SCIENTIFIC DATA ( 影响因子:6.9; 五年影响因子:8.7 )
ISSN:
年卷期: 2025 年 12 卷 1 期
页码:
收录情况: SCI
摘要: Yanbian cattle are a native cattle breed originating from the Yanbian Korean Autonomous Prefecture in Jilin Province, northeastern China. Developed over centuries through natural and artificial selection under cold climatic conditions, this breed is highly adapted to harsh environments, including low temperatures, rugged terrain, and sparse forage. Here, we constructed a high-quality chromosome-level genome assembly for Yanbian cattle using HiFi, Hi-C and RNA-seq data. The genome sequence was anchored to 30 chromosomes, with a total genome length of 2.8 Gb, a contig N50 of 86.41 Mb and a scaffold N50 of 111.08 Mb. Short-read data showed the average 99.56% mapping ratio to the assembly, validating base-level accuracy. Also, 93% complete BUSCOs verified the integrity of the assembled genome. 51.94% repetitive elements of the genome and 20,421 protein-coding genes were annotated. This Yanbian cattle genome serves as an indispensable resource for bovine genomic studies and local breed conservation, enabling both evolutionary insights and genetic characteristic analyses.
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