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Virus-Like Particles Based on the Novel Goose Parvovirus (NGPV) VP2 Protein Protect Ducks against NGPV Challenge

文献类型： 外文期刊

作者： Shang, Yu 1 ; Ma, Yao 1 ; Tang, Sheng 1 ; Chen, Xing 3 ; Feng, Helong 1 ; Li, Li 1 ; Wang, Hongcai 1 ; Zeng, Zhe 1 ; Yao, Lun 1 ; Zhang, Tengfei 1 ; Zeng, Chi 2 ; Luo, Qingping 1 ; Wen, Guoyuan 1 ;

作者机构： 1.Hubei Acad Agr Sci, Inst Anim Husb & Vet, Key Lab Prevent & Control Agents Anim Bacteriosis, Minist Agr & Rural Affairs,Hubei Prov Key Lab Anim, Wuhan 430064, Peoples R China

2.Wuhan Polytech Univ, Sch Life Sci & Technol, Wuhan 430023, Peoples R China

3.Wuhan Acad Agr Sci, Inst Anim Husb & Vet, Wuhan 430071, Peoples R China

4.Peoples Govt Hubei Prov, Hubei Hongshan Lab, Wuhan 430070, Peoples R China

关键词： novel goose parvovirus; VP2 protein; virus-like particles; immunogenicity

期刊名称：VACCINES （ 2022影响因子：7.8； 五年影响因子：7.4 ）

ISSN：

年卷期： 2023 年 11 卷 12 期

页码：

收录情况： SCI

摘要： Novel goose parvovirus (NGPV), a genetic variant of goose parvovirus, has been spreading throughout China since 2015 and mainly infects ducklings with the symptoms of growth retardation, beak atrophy, and protruding tongue, leading to huge economic losses every year. A safe and effective vaccine is urgently needed to control NGPV infection. In this study, virus-like particles (VLPs) of NPGV were assembled and evaluated for their immunogenicity. The VP2 protein of NGPV was expressed in Spodoptera frugiperda insect cells using baculovirus as vector. The VP2 protein was efficiently expressed in the nucleus of insect cells, and the particles with a circular or hexagonal shape and a diameter of approximately 30 nm, similar to the NGPV virion, were observed using transmission electron microscopy (TEM). The purified particles were confirmed to be composed of VP2 using western blot and TEM, indicating that the VLPs of NGPV were successfully assembled. Furthermore, the immunogenicity of the VLPs of NGPV was evaluated in Cherry Valley ducks. The level of NGPV serum antibodies increased significantly at 1-4 weeks post-immunization. No clinical symptoms or deaths of ducks occurred in all groups after being challenged with NGPV at 4 weeks post-immunization. There was no viral shedding in the immunized group. However, viral shedding was detected at 3-7 days post-challenge in the non-immunized group. Moreover, VLPs can protect ducks from histopathological lesions caused by NGPV and significantly reduce viral load in tissue at 5 days post-challenge. Based on these findings, NGPV VLPs are promising candidates for vaccines against NGPV.

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