文献类型： 外文期刊
作者： Du, Mengdan 1 ; Yang, Hailin 3 ; Song, Xueru 3 ; Li, Zhenggang 1 ; She, Xiaoman 1 ; Yu, Lin 1 ; Lan, Guobing 1 ; Ding, Shanwen 1 ; He, Zifu 1 ; Tang, Yafei 1 ;
作者机构： 1.Guangdong Acad Agr Sci, Inst Plant Protect, Key Lab Green Prevent & Control Fruits & Vegetable, Guangdong Prov Key Lab High Technol Plant Protect,, Guangzhou 510640, Peoples R China
2.South China Agr Univ, Coll Plant Protect, Guangzhou, Peoples R China
3.Yunnan Tobacco Co, Engn Ctr Biol Control Dis & Pests Tobacco Ind, Yuxi Branch, Yuxi, Peoples R China
关键词： Conyza canadensis; new host; tobacco leaf curl Yunnan virus
期刊名称：JOURNAL OF PHYTOPATHOLOGY （ 影响因子：1.5； 五年影响因子：1.8 ）
ISSN： 0931-1785
年卷期： 2024 年 172 卷 3 期
页码：
收录情况： SCI
摘要： In April 2023, leaf curl, crinkling symptoms were found on Conyza canadensis plants around tobacco fields located at Yuxi, Yunan province, China. PCR detection with genus-specific primers showed that symptomatic C. canadensis plants was infected by begomovirus. The full-length viral components and satellite molecules from the diseased C. canadensis (YN-2023) were obtained by enrichment of circular DNA with rolling circle amplification, restriction enzyme digestion, cloning, and DNA sequencing. The viral component (2, 750 nt, OR509733) shared the highest nt identity at 92.4% to tobacco leaf curl Yunnan virus isolate YN5741 (TbLCYNV-YN5741 [CN:Yn:Age:16], MN218671). A satellite molecule (1, 339 nt, OR509734) shared the highest nt identity at 86.5% to tobacco curly shoot betasatellite isolate Y115 (TbCSB-Y115 [CN:Yn:03], AJ457822). Another satellite molecule (1, 357 nt, OR509735) shared the highest nt identity at 94.2% to ageratum yellow vein Singapore alphasatellite (AYVSGA-[Singapore:1998], NC003414). Based on the threshold for classification of Begomovirus, Betasatellite, Alphasatellite, YN-2023 was a new strain of TbLCYNV and associated with TbCSB and AYVSGA. To our best knowledge, this is the first report that C. canadensis is a natural host of TbLCYNV.
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