文献类型： 外文期刊
作者： Fan, Xiangyun 1 ; Jiang, Peng 1 ; He, Yi 1 ; Zhang, Xu 1 ; Yu, Guihong 1 ; Zhou, Meixue 3 ; Zhang, Peng 1 ;
作者机构： 1.Jiangsu Acad Agr Sci, Inst Food Crops, Nanjing 210014, Jiangsu, Peoples R China
2.Zhongshan Biol Breeding Lab, Nanjing 210014, Jiangsu, Peoples R China
3.Univ Tasmania, Tasmanian Inst Agr, Hobart, Tas, Australia
关键词： Common wheat; Grain hardness; GWAS; QTL; KASP marker
期刊名称：BMC PLANT BIOLOGY （ 影响因子：4.8； 五年影响因子：5.4 ）
ISSN： 1471-2229
年卷期： 2024 年 24 卷 1 期
页码：
收录情况： SCI
摘要： Grain hardness has important effects on grain quality and the end-use of wheat. In this study, a collection of 103 common wheat germplasms and a DH population of 194 lines were used to identify new quantitative trait loci (QTL) for grain hardness. Two stable genetic loci on chromosome 5D were detected under different locations and years with one of them being the Ha locus on 5DS where the major gene Puroindolines for wheat grain hardness is located. Another locus of qTaHa-5DL also showed a significant impact on grain hardness index (HI) with HI increasing from similar to 20 to similar to 45 and hardness type changing from soft to mixed when Puroindolines are wild type. A kompetitive allele-specific PCR (KASP) marker for qTaHa-5DL was developed and the effectiveness of the QTL was confirmed in 184 breeding lines with the marker K-Ha5DL dividing wheat into two distinct categories. This new QTL can be effectively used to select soft or medium hard wheat.
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