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Construction and optimization of the TRV-mediated VIGS system in Areca catechu embryoids

文献类型： 外文期刊

作者： Yang, Xingqi 1 ; Liu, Zijia 1 ; Li, Yu 1 ; Zou, Jixin 2 ; Zheng, Yusheng 1 ; Li, Dongdong 1 ;

作者机构： 1.Hainan Univ, Sanya Nanfan Res Inst, Coll Trop Crops, Hainan 570228, Peoples R China

2.Chinese Acad Trop Agr Sci CATAS, Rubber Res Inst, Haikou, Peoples R China

关键词： Areca catechu; Callus; Virus-induced gene silencing; Phytoene desaturase gene; Gene function analysis

期刊名称：SCIENTIA HORTICULTURAE （ 影响因子：4.2； 五年影响因子：4.6 ）

ISSN： 0304-4238

年卷期： 2024 年 338 卷

页码：

收录情况： SCI

摘要： Areca catechu, a member of the palm family Arecaceae native to tropical regions, holds significant economic importance as a tropical crop. This study explores the application of virus-induced gene silencing (VIGS) in A. catechu embryogenic callus tissues, utilizing the phytoene desaturase gene (AcPDS) of A. catechu as a reporter gene. Results demonstrate that employing EHA105 strain, an Agrobacterium infection liquid concentration of OD600 = 0.5, an infection duration of 5 min, acetosyringone (AS) concentration of 21.5 mg/L, co-cultivation at 19(degrees)C for 2 days followed by 28 degrees C for 3 days, and a subsequent 14-day post-co-cultivation period, the experimental group (pTRV1+pTRV2-AcPDS) exhibited significantly reduced gene expression compared to the control group CK (pTRV1+pTRV2), manifesting the anticipated photobleaching phenotype by the 21st day. RT-PCR analysis revealed that AcPDS gene expression in TRV-mediated photobleached A. catechu embryos decreased to 0.227 times that of the control group CK. This established VIGS system in A. catechu embryoids establishes an effective experimental platform for studying functional genes in A. catechu.

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