Highly Efficient Agrobacterium tumefaciens Mediated Transformation of Oil Palm Using an EPSPS-Glyphosate Selection System
文献类型： 外文期刊
作者： Chao, Ouyang 1 ; Jin, Xiongxia 2 ; Guo, Qinghui 1 ; Luo, Shaojie 3 ; Zheng, Yusheng 3 ; Zou, Jixin 1 ; An, Baoguang 5 ; Li, Dongdong 1 ;
作者机构： 1.Hainan Univ, Coll Trop Agr & Forestry, Natl Key Lab Trop Crop Breeding, Sanya 572025, Peoples R China
2.Hainan Univ, Coll Trop Agr & Forestry, Haikou 570228, Peoples R China
3.Hainan Univ, Sch Life & Hlth Sci, Haikou 570228, Peoples R China
4.Chinese Acad Trop Agr Sci, Coconut Res Inst, Wenchang 571339, Peoples R China
5.Guangdong Bolian Biotechnol Co Ltd, Guangzhou 511466, Peoples R China
关键词： oil palm; genetic transformation; embryonic callus; EPSPS; glyphosate; Agrobacterium-mediated; mScarlet-I
期刊名称：PLANTS-BASEL （ 影响因子：4.1； 五年影响因子：4.5 ）
ISSN： 2223-7747
年卷期： 2024 年 13 卷 23 期
页码：
收录情况： SCI
摘要： Oil palm (Elaeis guineensis Jacq.) is the most efficient oil-producing crop globally, yet progress in its research has been hampered by the lack of effective genetic transformation systems. The EPSPS gene, encoding 5-enolpyruvylshikimate-3-phosphate synthase, has been used as a transgenic selection marker in various crops, including rice and soybean. This study evaluated the EPSPS/glyphosate selection system for oil palm transformation. We constructed a binary expression vector, pCGlyDESCLI-C, containing the TIPS-EiEPSPS selection marker from goosegrass and the mScarlet-I red fluorescent reporter gene. This vector was introduced into oil palm embryonic callus (EC) via Agrobacterium-mediated transformation. After optimizing the transformation steps, positive calli were obtained, and integration of the foreign gene into the oil palm genome was confirmed through molecular analysis. Notably, the selection efficiency of the EPSPS/glyphosate selection system exceeded that of the traditional hpt/hygromycin selection system, demonstrating its advantages. Our findings support the effectiveness of the TIPS-EiEPSPS/glyphosate selection system for oil palm genetic transformation, marking its first application in this species and offering a valuable tool for advancing research on this economically significant crop.
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