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A novel soybean malectin-like receptor kinase-encoding gene, GmMLRK1, provides resistance to soybean mosaic virus

文献类型： 外文期刊

作者： Che, Zhijun 1 ; Zhang, Shuyu 1 ; Pu, Yixiang 1 ; Yang, Yuming 1 ; Liu, Hailun 5 ; Yang, Hui 2 ; Wang, Li 1 ; Zhang, Yuhang 2 ; Liu, Baohui 2 ; Zhang, Hengyou 6 ; Wang, Hui 1 ; Cheng, Hao 1 ; Yu, Deyue 1 ;

作者机构： 1.Nanjing Agr Univ, Natl Ctr Soybean Improvement, Jiangsu Collaborat Innovat Ctr Modern Crop Prod, Natl Key Lab Crop Genet & Germplasm Enhancement, Nanjing, Peoples R China

2.Guangzhou Univ, Sch Life Sci, Guangzhou, Peoples R China

3.Ningxia Univ, Sch Agr, Yinchuan, Peoples R China

4.Henan Agr Univ, Coll Agron, Collaborat Innovat Ctr Henan Grain Crops, Zhengzhou, Peoples R China

5.Guangdong Acad Agr Sci, Inst Fruit Tree Res, Guangzhou, Peoples R China

6.Chinese Acad Sci, Northeast Inst Geog & Agroecol, Key Lab Soybean Mol Design & Breeding, Harbin, Peoples R China

关键词： Disease rate; functional study; genome-wide association study; GmMLRK1; malectin-like receptor kinase; soybean; soybean mosaic virus

期刊名称：JOURNAL OF EXPERIMENTAL BOTANY （ 影响因子：6.9； 五年影响因子：8.0 ）

ISSN： 0022-0957

年卷期： 2023 年 74 卷 8 期

页码：

收录情况： SCI

摘要： The malectin-like receptor kinase-encoding gene GmMLRK1was identified via a genome-wide association study and was proven to play a role in defense against soybean mosaic virus in soybean. Soybean mosaic virus (SMV) severely damages soybean [Glycine max (L.) Merr.] yield and seed quality. Moreover, the underlying genetic determinants of resistance to SMV remain largely unknown. Here, we performed a genome-wide association study (GWAS) of SMV resistance in a panel of 219 diverse soybean accessions across four environments and identified a new resistance-related gene, GmMLRK1, at the major resistance locus Rsv4 on chromosome 2. GmMLRK1 encodes a malectin-like receptor kinase (RK) that was induced earlier and to a greater degree in leaves of the SMV-resistant cultivar Kefeng No. 1 than in those of the susceptible cultivar Nannong 1138-2 after inoculation. We demonstrated that soybean plants overexpressing GmMLRK1 show broad-spectrum resistance to both strains SC7 and SC3 on the basis of reduced viral accumulation, increased reactive oxygen species production, and local cell death associated with the hypersensitive response. In contrast, GmMLRK1 knockout mutants were more susceptible to both pathotypes. Haplotype analysis revealed the presence of five haplotypes (H1-H5) within the soybean population, and only H1 provided SMV resistance, which was independent of its tightly linked SMV resistance gene RNase-H at the same locus. These results report a novel gene that adds new understanding of SMV resistance and can be used for breeding resistant soybean accessions.

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