Production of donor-derived offsprings by allogeneic transplantation of oogonia in the adult Japanese flounder (Paralichthys olivaceus)
文献类型： 外文期刊
作者： Ren, Yuqin 1 ; Sun, Zhaohui 2 ; Wang, Yufen 2 ; Yu, Qinghai 2 ; Wang, Guixing 2 ; He, Zhongwei 2 ; Liu, Yufeng 2 ; Jiang, 1 ;
作者机构： 1.Hebei Univ, Coll Life Sci, Baoding 071000, Peoples R China
2.Chinese Acad Fishery Sci, Beidaihe Cent Expt Stn, Qinhuangdao 066100, Hebei, Peoples R China
关键词： Germline stem cells; Germ cell transplantation; Spermatogenesis; Vasa; Paralichthys olivaceus
期刊名称：AQUACULTURE （ 影响因子：4.242； 五年影响因子：4.723 ）
ISSN： 0044-8486
年卷期： 2021 年 543 卷
页码：
收录情况： SCI
摘要： Germ cell transplantation (GCT) is a promising reproductive technology in animals, including teleost fish. Here, we established a GCT procedure in Japanese flounder (Paralichthys olivaceus). Two-year-old male Japanese flounder were treated with temperature (28 degrees C) and busulfan (two doses, 18 mg/kg) to deplete endogenous germ cells and were then used as recipients. The discontinuous Percoll gradient purified oogonia were used as donor germ cells and transplanted into the recipients' testes through the genital pore. Histological and fluorescent observations showed the colonization, proliferation, and differentiation of transplanted oogonia in the testes of recipient fish. Ten months after GCT, 16 of 17 recipients produced sperm, and among them, two recipients produced donor-derived sperm, which was confirmed by microsatellite genotyping. The sperm of these two recipients were used to fertilize the eggs of wild-type females, and the donor-derived germline transmission rates were 45.5% and 43.0%, respectively, based on the microsatellite paternity analysis of the hatched larvae. Our established protocol can be applied to accelerate the reproduction cycle and be used for generating valuable surrogate breeding material and conserving the germplasm resources of Japanese flounder.
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