您好，欢迎访问中国水产科学研究院 机构知识库！
中国水产科学研究院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Characterization of kiss2/kissr2 system in largemouth bass (Micropterus salmoides) and Kiss2-10 peptide regulation of the hypothalamic-pituitary-gonadal axis

文献类型： 外文期刊

作者： Li, Wuhui 1 ; Hu, Jie 1 ; Sun, Chengfei 1 ; Dong, Junjian 1 ; Liu, Zhigang 1 ; Yuan, Ju 1 ; Tian, Yuanyuan 1 ; Zhao, Jinliang 3 ; Ye, Xing 1 ;

作者机构： 1.Chinese Acad Fishery Sci, Key Lab Trop & Subtrop Fishery Resource Applicat, Key Lab Aquat Anim Immune Technol Guangdong Prov, Pearl River Fisheries Res Inst,Minist Agr & Rural, Guangzhou 510380, Guangdong, Peoples R China

2.Hunan Normal Univ, State Key Lab Dev Biol Freshwater Fish, Changsha 410081, Peoples R China

3.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Shanghai 201306, Peoples R China

关键词： Micropterus salmoides; Kiss2/kissr2; Expression pattern; Kisspeptin administration; Gonadal development

期刊名称：COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY （ 影响因子：2.495； 五年影响因子：2.567 ）

ISSN： 1096-4959

年卷期： 2022 年 257 卷

页码：

收录情况： SCI

摘要： The kisspeptin system, which lies upstream of the hypothalamic-pituitary-gonadal (HPG) axis, is believed to function as a regulator of reproduction in teleosts. In this study, we isolated and characterized kiss2 and its receptor kissr2 in largemouth bass (Micropterus salmoides). The complete coding sequences of kiss2 and kissr2 were 375 and 1134 bp long and encoded precursor proteins 124 and 377 amino acid long, respectively. Real-time PCR showed that kiss2 and kissr2 were primarily expressed in the HPG axis. The expression profile of kiss2 and kissr2 varied with gonadal development, with the highest and lowest expression levels being detected during the immature and final maturation stages, respectively. Intraperitoneal injection of exogenous Kiss2-10 peptide increased the transcript levels of gnrh3, kissr2, fsh beta, lh beta, ar, and er2 within 24 h (p < 0.05), as well as plasma levels of 17 beta-estradiol and testosterone. Histological analysis indicated that chronic administration of exogenous Kiss2-10 peptide accelerated vitellogenesis in females and spermatogenesis in males. Further, in situ hybridization revealed that kiss2 is expressed in the ooplasm and vitelline envelope of oocytes and the spermatocytes of testes. In addition, experiments using gonad tissue primary cell cultures indicated that exogenous Kiss2-10 peptide stimulates the expression of reproduction-related genes. Collectively, our findings indicate that the kiss2/kissr2 system in largemouth bass is involved in regulating gonadal development through the HPG axis.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：中国水产科学研究院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台