OsGLYI3, a glyoxalase gene expressed in rice seed, contributes to seed longevity and salt stress tolerance
文献类型： 外文期刊
作者： Liu, Shengjie 1 ; Liu, Wenhua 2 ; Lai, Jianyun 2 ; Liu, Qinjian 2 ; Zhang, Wenhu 2 ; Chen, Zhongjian 2 ; Gao, Jiadong 2 ; Song, Songquan 3 ; Liu, Jun 2 ; Xiao, Yinghui 1 ;
作者机构： 1.Hunan Agr Univ, Coll Agron, Changsha 410128, Peoples R China
2.Guangdong Acad Agr Sci, Agrobiol Gene Res Ctr, Guangdong Key Lab Crop Germplasm Resources Preserv, Guangzhou 510640, Peoples R China
3.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
关键词： Glyoxalase I; Methylglyoxal; Stress tolerance; Seed longevity; Rice(Oryza sativa L; )
期刊名称：PLANT PHYSIOLOGY AND BIOCHEMISTRY （ 影响因子：5.437； 五年影响因子：5.731 ）
ISSN： 0981-9428
年卷期： 2022 年 183 卷
页码：
收录情况： SCI
摘要： The glyoxalase pathway plays a vital role in the chemical detoxification of methylglyoxal (MG) in biological systems. Our previous study suggested that OsGLYI3 may be effective in seed natural aging. In this study, the rice OsGLYI3 gene was cloned and characterized as specifically expressed in the seed. The accelerated aging (AA) treatment results indicated significant roles of OsGLYI3 in seed longevity and vigor, as the seeds of the transgenic lines with overexpressed and knocked-out OsGLYI3 exhibited higher and lower germination, respectively. The AA treatment also increased the superoxide dismutase (SOD) activity in the overexpressed transgenic seeds compared to the wild-type seeds yet lowered the SOD activity in the CRISPR/Cas9-derived transgenic rice lines. Rice OsGLYI3 was markedly upregulated in response to NaCl induced stress conditions. Compared to wild-type plants, overexpressed transgenic rice lines exhibited increased GLYI activity, decreased MG levels and improved salt stress tolerance, while CRISPR/Cas9 knockout transgenic rice lines showed decreased glyoxalase I activity, increased MG levels, and greater sensitivity to stress treatments with NaCl. Collectively, our results confirmed for the first time that OsGLYI3 is specifically expressed in rice seeds and contributes to seed longevity and salt stress tolerance.
- 相关文献
作者其他论文 更多>>
-
The Malectin-like kinase gene MdMDS1 negatively regulates the resistance of Pyrus betulifolia to Valsa canker by promoting the expression of PbePME1
作者：Zheng, Yan;Zhao, Dan;Lu, Yuan;Liu, Zhihong;Sun, E.;Yu, Hongqiang;Mao, Xia;Cai, Minrui;Zuo, Cunwu;Chen, Zhongjian;Zuo, Cunwu
关键词：
-
The Characterization of the Purine Nucleoside Phosphorylase from Agaricus bisporus and Its Potential Application in Reducing Purine Content in Beer
作者：Liu, Jun;Lu, Jian;Liu, Jun;Lu, Jian;Liu, Jun;Lu, Jian;Liu, Jun;Liu, Jun
关键词：beer; gout; purines; Agaricus bisporus; purine nucleoside phosphorylase
-
Integrated metabolomic and transcriptomic analysis of Pogostemon cablin shed new light on the complete biosynthesis pathway of pogostone
作者：Wang, Sen;Zeng, Zhaoqi;Zhang, Qi;Liu, Wenhua;Liu, Qinjian;Xie, Chong;Bei, Jinlong;Chen, Bingxian;Zhang, Aixia;Zeng, Zhaoqi;Xie, Chong
关键词：patchoulol; pogostone; biosynthesis; key enzyme; transcriptomics; patchouli; metabolomics
-
Pretreatment with nano-silver extends the post-harvest longevity of gladiolus cut flowers by reducing free water mobility
作者：Li, Xiumei;Liu, Qinjian;Chen, Yuanyuan;Chen, Zhongjian;Liu, Jun;Yan, Shijuan;Li, Hongbo;He, Shenggen
关键词：Water state; Nanoparticles; Vase life; Nuclear magnetic resonance; Cut flowers
-
pH/Enzyme-responsive nanocarrier pesticide delivery system: Realizing targeted release in pest gut for enhanced Apis cerana safety
作者：Du, Pengrui;Wu, Hao;Hou, Ruiquan;Liu, Jun;Chang, Jinzhe;Miao, Xiaoran;Wang, Xin;Liu, Pengpeng;Zhu, Shiqi;Zhang, Zhixiang;Zhang, Peiwen;Shao, Xuehua;Kaziem, Amir E.;Zhang, Peiwen
关键词：Zein; O-carboxymethyl chitosan; Environmentally responsive; Controlled-release pesticide; Biological safety
-
Assembly and analysis of the complete mitochondrial genome of Leonurus japonicus (Lamiaceae)
作者：Hu, Qun;Li, Qing;Luo, Yongjian;Zhang, Wenhu;Hu, Qun;Mao, Yin;Deng, Zhijun;Hu, Qun;Mao, Yin;Deng, Zhijun
关键词：
Leonurus japonicus Houtt; Mitochondrial genome; RNA editing site; Repeat sequence -
Strategies for Reducing Purine Accumulation in Beer: From Metabolic Mechanisms to Brewing Technology Innovations
作者：Liu, Jun;Lu, Jian;Liu, Jun;Lu, Jian;Liu, Jun;Lu, Jian;Liu, Jun;Liu, Jun
关键词：low-purine beer; hyperuricemia; purine metabolism; brewing biotechnology; yeast engineering
