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A conserved Pol II elongator SPT6L mediates Pol V transcription to regulate RNA-directed DNA methylation in Arabidopsis

文献类型： 外文期刊

作者： Liu, Yujuan 1 ; Shu, Jie 2 ; Zhang, Zhi 1 ; Ding, Ning 2 ; Liu, Jinyuan 1 ; Liu, Jun 5 ; Cui, Yuhai 7 ; Wang, Changhu 1 ; Chen, Chen 1 ;

作者机构： 1.Chinese Acad Sci, State Key Lab Plant Divers & Specialty Crops, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

2.Chinese Acad Sci, Key Lab South China Agr Plant Mol Anal & Genet Imp, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

3.Chinese Acad Sci, Guangdong Prov Key Lab Appl Bot, South China Bot Garden, Guangzhou 510650, Guangdong, Peoples R China

4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China

5.Guangdong Acad Agr Sci, Guangzhou 510640, Guangdong, Peoples R China

6.Lanzhou Univ, Sch Life Sci, MOE Key Lab Cell Act & Stress Adaptat, Lanzhou 730000, Peoples R China

7.Agr & Agri Food Canada, London Res & Dev Ctr, London, ON N5V 4T3, Canada

8.Western Univ, Dept Biol, London, ON N6A 5B7, Canada

期刊名称：NATURE COMMUNICATIONS （ 影响因子：14.7； 五年影响因子：16.1 ）

ISSN：

年卷期： 2024 年 15 卷 1 期

页码：

收录情况： SCI

摘要： In plants, the plant-specific RNA polymerase V (Pol V) transcripts non-coding RNAs and provides a docking platform for the association of accessory proteins in the RNA-directed DNA methylation (RdDM) pathway. Various components have been uncovered that are involved in the process of DNA methylation, but it is still not clear how the transcription of Pol V is regulated. Here, we report that the conserved RNA polymerase II (Pol II) elongator, SPT6L, binds to thousands of intergenic regions in a Pol II-independent manner. The intergenic enrichment of SPT6L, interestingly, co-occupies with the largest subunit of Pol V (NRPE1) and mutation of SPT6L leads to the reduction of DNA methylation but not Pol V enrichment. Furthermore, the association of SPT6L at Pol V loci is dependent on the Pol V associated factor, SPT5L, rather than the presence of Pol V, and the interaction between SPT6L and NRPE1 is compromised in spt5l. Finally, Pol V RIP-seq reveals that SPT6L is required to maintain the amount and length of Pol V transcripts. Our findings thus uncover the critical role of a Pol II conserved elongator in Pol V mediated DNA methylation and transcription, and shed light on the mutual regulation between Pol V and II in plants.

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