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Lacticaseibacillus casei ATCC 393 Cannot Colonize the Gastrointestinal Tract of Crucian Carp

文献类型： 外文期刊

作者： Zhang, Hongyu 1 ; Mu, Xiyan 1 ; Wang, Hongwei 2 ; Wang, Haibo 1 ; Wang, Hui 1 ; Li, Yingren 1 ; Mu, Yingchun 2 ; Song, Jinlong 2 ; Xia, Lei 2 ;

作者机构： 1.Chinese Acad Fishery Sci, Fishery Resource & Environm Res Ctr, Beijing 100141, Peoples R China

2.Chinese Acad Fishery Sci, Beijing 100141, Peoples R China

3.Chinese Acad Fishery Sci, Minist Agr & Rural Affairs, Key Lab Control Qual & Safety Aquat Prod, Beijing 100141, Peoples R China

关键词： Lacticaseibacillus casei; colonization; crucian carp; gastrointestinal tract; Co-60 irradiation sterilization; transit marker; Geobacillus stearothermophilus; high-throughput sequencing

期刊名称：MICROORGANISMS （ 影响因子：4.926； 五年影响因子：5.143 ）

ISSN：

年卷期： 2021 年 9 卷 12 期

页码：

收录情况： SCI

摘要： Lactic acid bacteria (LAB) are commonly applied to fish as a means of growth promotion and disease prevention. However, evidence regarding whether LAB colonize the gastrointestinal (GI) tract of fish remains sparse and controversial. Here, we investigated whether Lacticaseibacillus casei ATCC 393 (Lc) can colonize the GI tract of crucian carp. Sterile feed irradiated with Co-60 was used to eliminate the influence of microbes, and 100% rearing water was renewed at 5-day intervals to reduce the fecal-oral circulation of microbes. The experiment lasted 47 days and was divided into three stages: the baseline period (21 days), the administration period (7 days: day -6 to 0) and the post-administration period (day 1 to 19). Control groups were fed a sterile basal diet during the whole experimental period, whereas treatment groups were fed with a mixed diet containing Lc (1 x 10(7) cfu/g) and spore of Geobacillus stearothermophilus (Gs, 1 x 10(7) cfu/g) during the administration period and a sterile basal diet during the baseline and post-administration periods. An improved and highly sensitive selective culture method (SCM) was employed in combination with a transit marker (a Gs spore) to monitor the elimination of Lc in the GI tract. The results showed that Lc (<2 cfu/gastrointestine) could not be detected in any of the fish sampled from the treatment group 7 days after the cessation of the mixed diet, whereas Gs could still be detected in seven out of nine fish at day 11 and could not be detected at all at day 15. Therefore, the elimination speed of Lc was faster than that of the transit marker. Furthermore, high-throughput sequencing analysis combined with SCM was used to reconfirm the elimination kinetics of Lc in the GI tract. The results show that the Lc in the crucian carp GI tract, despite being retained at low relative abundance from day 7 (0.11% +/- 0.03%) to 21, was not viable. The experiments indicate that Lc ATCC 393 cannot colonize the GI tract of crucian carp, and the improved selective culture in combination with a transit marker represents a good method for studying LAB colonization of fish.

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