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The Septin Gene StSep4 Contributes to the Pathogenicity of Setosphaeria turcica by Regulating the Morphology, Cell Wall Integrity, and Pathogenic Factor Biosynthesis

文献类型： 外文期刊

作者： Li, Pan 1 ; Sun, Hehe 3 ; Han, Xinpeng 3 ; Long, Feng 3 ; Shen, Shen 3 ; Li, Zhiyong 4 ; Zeng, Fanli 3 ; Hao, Zhimin 1 ; Dong, Jingao 1 ;

作者机构： 1.Hebei Agr Univ, State Key Lab North China Crop Improvement, Baoding 071001, Hebei, Peoples R China

2.Hebei Agr Univ, Coll Plant Protect, Baoding 071001, Hebei, Peoples R China

3.Hebei Agr Univ, Coll Life Sci, Baoding 071001, Hebei, Peoples R China

4.Hebei Acad Agr & Forestry Sci, Inst Millet Crops, Shijiazhuang 050035, Hebei, Peoples R China

5.Hebei Agr Univ, Collegeof Life Sciences,, Baoding 071001, Hebei, Peoples R China

关键词： Setosphaeriaturcica; forchlorfenuron; septin; StSep4; pathogenicity

期刊名称：JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY （ 影响因子：6.1； 五年影响因子：6.3 ）

ISSN： 0021-8561

年卷期： 2023 年 71 卷 49 期

页码：

收录情况： SCI

摘要： Septins are a conserved group of GTP-binding proteins found in all eukaryotes and are the fourth-most abundant cytoskeletal proteins. Septins of some pathogenic fungi are involved in morphological changes related to infection. Our previous studies have identified four core septins (StSep1-4) in Setosphaeria turcica, the causal agent of northern corn leaf blight, while only StSep4 is significantly upregulated during the invasive process. We therefore used forchlorfenuron (FCF), the specific inhibitor of septin, and Delta StSep4 knockout mutants to further clarify the role of septins in S. turcica pathogenicity. FCF treatment caused a dose-dependent reduction in S. turcica colony growth, delayed the formation of infection structures, and reduced the penetration ability. Delta StSep4 knockout mutants displayed abnormal mycelium morphology, slow mycelial growth, conidiation deficiency, delayed appressorium development, and weakened pathogenicity. StSep4 deletion also broke cell wall integrity, altered chitin distribution, decreased the melanin content, and disrupted normal nuclear localization. A transcriptomic comparison revealed that genes differentially expressed between Delta StSep4 and WT were enriched in terms of ribosomes, protein translation, membrane components, and transmembrane transport activities. Our results demonstrate that StSep4 is required for morphology and pathogenicity in S. turcica, making it a promising target for the development of novel fungicides.

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