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The genome sequencing and comparative genomics analysis of Rhizoctonia solani reveals a novel effector family owning a uinque domain in Basidiomycetes

文献类型： 外文期刊

作者： Liu, Yuwei 1 ; Liu, Jiayue 1 ; Sun, Mingqing 3 ; Mao, Yanan 1 ; Feng, Shang 1 ; Shen, Shen 1 ; Liu, Tingting 3 ; Cao, Zhiyan 1 ; Li, Zhiyong 2 ; Hao, Zhimin 1 ; Dong, Jingao 1 ;

作者机构： 1.Hebei Agr Univ, Hebei Bioinformat Utilizat & Technol Innovat Ctr A, State Key Lab North China Crop Improvement & Regul, Hebei 071001, Peoples R China

2.Minist Agr & Rural Affairs, Hebei Acad Agr & Forestry Sci, Inst Millet Crops, Key Lab Genet Improvement & Utilizat Featured Coar, Shijiazhuang 050035, Hebei, Peoples R China

3.Shijiazhuang Agr Technol Extens Ctr, Shijiazhuang 050051, Peoples R China

4.Hebei Agr Univ, Coll Plant Protect, Hebei Key Lab Plant Physiol & Mol Pathol, Baoding 071001, Hebei, Peoples R China

关键词： Rhizoctonia solani; Genome sequencing; Comparative genomic analysis; Effector; dsRNA; Spray-induced gene silencing

期刊名称：INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES （ 影响因子：7.7； 五年影响因子：7.7 ）

ISSN： 0141-8130

年卷期： 2024 年 278 卷

页码：

收录情况： SCI

摘要： Rhizoctonia solani is a soil-borne pathogen with 14 anastomosis groups (AGs), and different subgroups are genetically diverse. However, the genetic factors contributing to the pathogenicity of the fungus have not been well characterized. In this study, the genome of R. solani AG1-ZJ was sequenced. As the result, a 41.57 Mb draft genome containing 12,197 putative coding genes was obtained. Comparative genomic analysis of 11 different AGs revealed conservation and unique characteristics between the AGs. Furthermore, a novel effector family containing a 68 amino acid conserved domain unique in basidiomycetous fungi was characterized. Two effectors containing the conserved domain in AG4-JY were identified, and named as RsUEB1 and RsUEB2. Furthermore, the spray-induced gene silencing strategy was used to generate a dsRNA capable of silencing the conserved domain sequence of RsUEB1 and RsUEB2. This dsRNA can significantly reduce the expression of RsUEB1 and RsUEB2 and the pathogenicity of AG4-JY on foxtail millet, maize, rice and wheat. In conclusion, this study provides significant insights into the pathogenicity mechanisms of R. solani. The identification of the conserved domain and the successful use of dsRNA silencing of the gene containing the conserved domain will offer a new strategy for controlling sheath blight in cereal crops.

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