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The bHLH transcription factor PPLS1 regulates the color of pulvinus and leaf sheath in foxtail millet (Setaria italica)

文献类型： 外文期刊

作者： Bai, Hui 1 ; Song, Zhenjun 1 ; Zhang, Yan 3 ; Li, Zhiyong 1 ; Wang, Yongfang 1 ; Liu, Xue 4 ; Ma, Jifang 1 ; Quan, Jianzh 1 ;

作者机构： 1.Hebei Acad Agr & Forestry Sci, Natl Foxtail Millet Improvement Ctr, Inst Millet Crops, Minor Cereal Crops Lab Hebei Prov, Shijiazhuang 050035, Hebei, Peoples R China

2.Hebei Normal Univ, Coll Life Sci, Shijiazhuang 050024, Hebei, Peoples R China

3.Nankai Univ, Coll Life Sci, Tianjin 300071, Peoples R China

4.Beijing Acad Agr & Forestry Sci, Beijing Vegetable Res Ctr, Natl Engn Res Ctr Vegetables, Beijing Key Lab Vegetable Germplasms Improvement, Beijing 100097, Peoples R China

5.Sichuan Agr Univ, Rice Res Inst, Chengdu 611130, Peoples R China

6.Shandong Normal Univ, Coll Life Sci, Jinan 250014, Peoples R China

关键词： Anthocyanin; bHLH transcription factor; Foxtail millet (Setaria italica); Leaf sheath; PPLS1; Pulvinus

期刊名称：THEORETICAL AND APPLIED GENETICS （ 影响因子：5.699； 五年影响因子：5.565 ）

ISSN： 0040-5752

年卷期： 2020 年 133 卷 6 期

页码：

收录情况： SCI

摘要： Key message The bHLH transcription factor, PPLS1, interacts with SiMYB85 to control the color of pulvinus and leaf sheath by regulating anthocyanin biosynthesis in foxtail millet (Setaria italica). Foxtail millet (Setaria italica), a self-pollinated crop with numerous small florets, is difficult for cross-pollination. The color of pulvinus and leaf sheath with purple being dominant to green is an indicative character and often used for screening authentic hybrids in foxtail millet crossing. Deciphering molecular mechanism controlling this trait would greatly facilitate genetic improvement of cultivars in foxtail millet. Here, using the F-2 bulk specific-locus amplified fragment sequencing approach, we mapped the putative causal gene for the purple color of pulvinus and leaf sheath (PPLS) trait to a 100 Kb region on chromosome 7. Expression analyses of the 15 genes in this region revealed that Seita.7G195400 (renamed here as PPLS1) was differentially expressed between purple and green cultivars. PPLS1 encodes a bHLH transcription factor and is localized in the nucleus with a transactivation activity. Furthermore, we observed that expression of a MYB transcription factor gene, SiMYB85 (Seita.4G086300) involved in anthocyanin biosynthesis, shows a totally positive association with that of PPLS1. Heterologous co-expression of both PPLS1 and SiMYB85 in tobacco leaves led to elevated anthocyanin accumulation and expression of some anthocyanin-related genes. Furthermore, PPLS1 physically interacts with SiMYB85. Taken together, our results suggest that PPLS1 interacts with SiMYB85 to control the color of pulvinus and leaf sheath by regulating anthocyanin biosynthesis in foxtail millet.

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