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Engineering crop Phytophthora resistance by targeting pathogen-derived PI3P for enhanced catabolism

文献类型： 外文期刊

作者： Yang, Kun 1 ; Yan, Qiang 1 ; Wang, Yi 1 ; Zhu, Wenyi 1 ; Wang, Xiaodan 3 ; Li, Xiaobo 4 ; Peng, Hao 5 ; Zhou, Yang 1 ; Jing, Maofeng 1 ; Dou, Daolong 1 ;

作者机构： 1.Nanjing Agr Univ, Acad Adv Interdisciplinary Studies, Coll Plant Protect, Key Lab Plant Immun, Nanjing 210095, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Ind Crops, Jiangsu Key Lab Hort Crop Genet Improvement, Nanjing 210095, Peoples R China

3.China Agr Univ, Coll Plant Protect, Beijing 100091, Peoples R China

4.Guangdong Acad Agr Sci, Crops Res Inst, Guangdong Prov Key Lab Crop Genet Improvement, Guangzhou 510640, Guangdong, Peoples R China

5.Washington State Univ, Dept Plant Pathol, Pullman, WA 99164 USA

关键词： Phytophthora resistance; PI3P; RxLR effector; transgenic plants

期刊名称：PLANT COMMUNICATIONS （ 影响因子：10.5； 五年影响因子：10.5 ）

ISSN： 2590-3462

年卷期： 2023 年 4 卷 2 期

页码：

收录情况： SCI

摘要： Phytophthora pathogens lead to numerous economically damaging plant diseases worldwide, including potato late blight caused by P. infestans and soybean root rot caused by P. sojae. Our previous work showed that Phytophthora pathogens may generate abundant phosphatidylinositol 3-phosphate (PI3P) to promote infection via direct association with RxLR effectors. Here, we designed a disease control strat-egy for metabolizing pathogen-derived PI3P by expressing secreted Arabidopsis thaliana phosphatidylino-sitol-4-phosphate 5-kinase 1 (AtPIP5K1), which can phosphorylate PI3P to PI(3,4)P2. We fused AtPIP5K1 with the soybean PR1a signal peptide (SP-PIP5K1) to enable its secretion into the plant apoplast. Trans -genic soybean and potato plants expressing SP-PIP5K1 showed substantially enhanced resistance to various P. sojae and P. infestans isolates, respectively. SP-PIP5K1 significantly reduced PI3P accumulation during P. sojae and soybean interaction. Knockout or inhibition of PI3 kinases (PI3Ks) in P. sojae compro-mised the resistance mediated by SP-PIP5K1, indicating that SP-PIP5K1 action requires a supply of pathogen-derived PI3P. Furthermore, we revealed that SP-PIP5K1 can interfere with the action of P. sojae mediated by the RxLR effector Avr1k. This novel disease control strategy has the potential to confer durable broad-spectrum Phytophthora resistance in plants through a clear mechanism in which catabolism of PI3P interferes with RxLR effector actions.

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