您好，欢迎访问江苏省农业科学院 机构知识库！
江苏省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Antiviral activity of Stephania japonica extract against porcine epidemic diarrhea virus infection

文献类型： 外文期刊

作者： Zhao, Yong 1 ; Fan, Baochao 1 ; Wang, Yi 1 ; Sun, Min 1 ; Guo, Rongli 1 ; Tang, Tao 5 ; Hu, Mi 1 ; Li, Bin 1 ;

作者机构： 1.Nanjing Forestry Univ, Coll Life Sci, Nanjing 210037, Peoples R China

2.Jiangsu Acad Agr Sci, Inst Vet Med, Nanjing 210014, Jiangsu, Peoples R China

3.Nanjing Agr Univ, Coll Vet Med, Nanjing 210095, Jiangsu, Peoples R China

4.Yangzhou Univ, Jiangsu Coinnovat Ctr Prevent & Control Important, Yangzhou 225009, Jiangsu, Peoples R China

5.Guotai Taizhou Ctr Technol Innovat Vet Biol, Taizhou 225300, Jiangsu, Peoples R China

6.Beijing Univ Agr, Coll Vet Med, Beijing 102206, Peoples R China

7.Minist Agr, Key Lab Vet Bioengn & Technol, Nanjing 210014, Jiangsu, Peoples R China

8.Minist Sci & Technol, Jiangsu Key Lab Food Qual & Safety, State Key Lab Cultivat Base, Nanjing 210014, Jiangsu, Peoples R China

9.Jiangsu Key Lab Zoonoses, Yangzhou 225009, Jiangsu, Peoples R China

关键词： PEDV; Cepharanthine extract; Bisbenzylisoquinoline alkaloids; Antiviral activity

期刊名称：VETERINARY MICROBIOLOGY （ 影响因子：2.7； 五年影响因子：2.9 ）

ISSN： 0378-1135

年卷期： 2025 年 308 卷

页码：

收录情况： SCI

摘要： Porcine epidemic diarrhea virus (PEDV) is a major cause of diarrhea in piglets, causing substantial economic losses to the global swine industry. At present, no specific antiviral medications are available to treat PEDV infections. Natural compounds, with their wide availability, diverse biological activities, and low toxicity, have emerged as promising candidates for antiviral drug discovery. This study screened eight plant-derived bisbenzylisoquinoline alkaloids, including cepharanthine and tetrandrine, to assess their anti-PEDV activity in vitro. The results demonstrated that cepharanthine extracts significantly reduced viral titers and genome copies, indicating strong anti-PEDV activity. Notably, the botanical extracts exerted antiviral effects at both the initial stage of infection and the late phase of virion release, as evidenced by reduced viral output and suppressed mRNA synthesis. Molecular docking and dynamic analyses revealed that cepharanthine binds to PEDV 3CLpro (Mpro) protease through hydrogen bonds and hydrophobic interactions, forming a stable complex. This interaction likely impairs Mpro function, thereby inhibiting viral replication and the synthesis of related proteins. In vivo experiments further confirmed that piglets treated with cepharanthine extracts exhibited significantly lower viral loads and better preservation of intestinal structure compared to the control group. These findings provide key insights into the antiviral effects of cepharanthine extracts, supporting their potential for further development as anti-PEDV therapies and as a foundation for plant-derived antiviral compound research.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：江苏省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台