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Microbial consortium assembly and functional analysis via isotope labelling and single-cell manipulation of polycyclic aromatic hydrocarbon degraders

文献类型： 外文期刊

作者： Li, Jibing 1 ; Luo, Chunling 1 ; Cai, Xixi 4 ; Zhang, Dayi 5 ; Guan, Guoqing 1 ; Li, Bei 7 ; Zhang, Gan 1 ;

作者机构： 1.Chinese Acad Sci, State Key Lab Organ Geochem, 511,Kehua St, Guangzhou 510640, Peoples R China

2.Chinese Acad Sci, Guangzhou Inst Geochem, Guangdong Hong Kong Macao Joint Lab Environm Pollu, 511,Kehua St, Guangzhou 510640, Peoples R China

3.Univ Chinese Acad Sci, Coll Resources & Environm, Beijing 100039, Peoples R China

4.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou 510640, Peoples R China

5.Jilin Univ, Key Lab Groundwater Resources & Environm, Minist Educ, Changchun 130012, Peoples R China

6.Jilin Univ, Coll New Energy & Environm, Changchun 130021, Peoples R China

7.Chinese Acad Sci, State Key Lab Appl Opt, Changchun Inst Opt Fine Mech & Phys, Changchun 130033, Peoples R China

8.HOOKE Instruments Ltd, Jilin Prov Raman Technol Engn Res Ctr, Changchun 130033, Peoples R China

关键词： in situ functional microbial consortia; Raman-activated cell sorting; stable-isotope probing; genome-directed cultivation; soil phenanthrene-degrading bacteria; single-cell genomic sequencing

期刊名称：ISME JOURNAL （ 影响因子：10.8； 五年影响因子：11.8 ）

ISSN： 1751-7362

年卷期： 2024 年 18 卷 1 期

页码：

收录情况： SCI

摘要： Soil microbial flora constitutes a highly diverse and complex microbiome on Earth, often challenging to cultivation, with unclear metabolic mechanisms in situ. Here, we present a pioneering concept for the in situ construction of functional microbial consortia (FMCs) and introduce an innovative method for creating FMCs by utilizing phenanthrene as a model compound to elucidate their in situ biodegradation mechanisms. Our methodology involves single-cell identification, sorting, and culture of functional microorganisms, resulting in the formation of a precise in situ FMC. Through Raman-activated cell sorting-stable-isotope probing, we identified and isolated phenanthrene-degrading bacterial cells from Achromobacter sp. and Pseudomonas sp., achieving precise and controllable in situ consortia based on genome-guided cultivation. Our in situ FMC outperformed conventionally designed functional flora when tested in real soil, indicating its superior phenanthrene degradation capacity. We revealed that microorganisms with high degradation efficiency isolated through conventional methods may exhibit pollutant tolerance but lack actual degradation ability in natural environments. This finding highlights the potential to construct FMCs based on thorough elucidation of in situ functional degraders, thereby achieving sustained and efficient pollutant degradation. Single-cell sequencing linked degraders with their genes and metabolic pathways, providing insights regarding the construction of in situ FMCs. The consortium in situ comprising microorganisms with diverse phenanthrene metabolic pathways might offer distinct advantages for enhancing phenanthrene degradation efficiency, such as the division of labour and cooperation or communication among microbial species. Our approach underscores the importance of in situ, single-cell precision identification, isolation, and cultivation for comprehensive bacterial functional analysis and resource exploration, which can extend to investigate MFCs in archaea and fungi, clarifying FMC construction methods for element recycling and pollutant transformation in complex real-world ecosystems.

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