SIP-metagenomics reveals key drivers of rhizospheric Benzo[a]pyrene bioremediation via bioaugmentation with indigenous soil microbes☆
文献类型： 外文期刊
作者： Zhao, Xuan 1 ; Cheng, Xianghui 1 ; Cai, Xixi 4 ; Wang, Shuang 1 ; Li, Jibing 1 ; Dai, Yeliang 1 ; Jiang, Longfei 1 ; Luo, Chunling 1 ; Zhang, Gan 1 ;
作者机构： 1.Chinese Acad Sci, Guangzhou Inst Geochem, State Key Lab Organ Geochem, Guangzhou 510640, Peoples R China
2.Chinese Acad Sci, Guangzhou Inst Geochem, Guangdong Hong Kong Macao Joint Lab Environm Pollu, Guangzhou 510640, Peoples R China
3.Kunming Univ, Coll Architecture & Civil Engn, Kunming 650214, Peoples R China
4.Guangdong Acad Agr Sci, Environm Hort Res Inst, Guangdong Key Lab Ornamental Plant Germplasm Innov, Guangzhou 510640, Peoples R China
5.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
6.Chengdu Technol Univ, Sch Mat & Environm Engn, Chengdu 611730, Peoples R China
关键词： Stable isotope probing (SIP); Bioaugmentation; Rhizoremediation; benzo[a]pyrene degrader; Metagenomic; Indigenous soil microbes
期刊名称：ENVIRONMENTAL POLLUTION （ 影响因子：7.6； 五年影响因子：8.3 ）
ISSN： 0269-7491
年卷期： 2024 年 360 卷
页码：
收录情况： SCI
摘要： Rhizoremediation and bioaugmentation have proven effective in promoting benzo[a]pyrene (BaP) degradation in contaminated soils. However, the mechanism underlying bioaugmented rhizospheric BaP degradation with native microbes is poorly understood. In this study, an indigenous BaP degrader (Stenotrophomonas BaP-1) isolated from petroleum-contaminated soil was introduced into ryegrass rhizosphere to investigate the relationship between indigenous degraders and rhizospheric BaP degradation. Stable isotope probing and 16S rRNA gene amplicon sequencing subsequently revealed 15 BaP degraders, 8 of which were directly associated with BaP degradation including Bradyrhizobium and Streptomyces. Bioaugmentation with strain BaP-1 significantly enhanced rhizospheric BaP degradation and shaped the microbial community structure. A correlation of BaP degraders, BaP degradation efficiency, and functional genes identified active degraders and genes encoding polycyclic aromatic hydrocarbon-ring hydroxylating dioxygenase (PAH-RHD) genes as the primary drivers of rhizospheric BaP degradation. Furthermore, strain BaP-1 was shown to not only engage in BaP metabolism but also to increase the abundance of other BaP degraders and PAH-RHD genes, resulting in enhanced rhizospheric BaP degradation. Metagenomic and correlation analyses indicated a significant positive relationship between glyoxylate and dicarboxylate metabolism and BaP degradation, suggesting a role for these pathways in rhizospheric BaP biodegradation. By identifying BaP degraders and characterizing their metabolic characteristics within intricate microbial communities, our study offers valuable insights into the mechanisms of bioaugmented rhizoremediation with indigenous bacteria for high-molecular-weight PAHs in petroleum-contaminated soils.
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