您好，欢迎访问广东省农业科学院 机构知识库！
广东省农业科学院机构知识库

全部

  • 全部
  • 标题
  • 学者
  • 机构
  • 关键词

Identification of key genes and pathways in duck fatty liver syndrome using gene set enrichment analysis

文献类型： 外文期刊

作者： Yang, Xue 1 ; Lin, Hao 1 ; Wang, Mengpan 1 ; Huang, Xuebing 1 ; Li, Kaichao 1 ; Xia, Weiguang 1 ; Zhang, Yanan 1 ; Wang, Shuang 1 ; Chen, Wei 1 ; Zheng, Chuntian 1 ;

作者机构： 1.Guangdong Acad Agr Sci, Minist Agr & Rural Affairs, State Key Lab Swine & Poultry Breeding Ind, Inst Anim Sci,Key Lab Anim Nutr & Feed Sci South C, Guangzhou 510640, Peoples R China

2.Anhui Sci & Technol Univ, Coll Anim Sci, Huainan 233100, Anhui, Peoples R China

3.Tianjin Agr Univ, Coll Anim Sci & Vet Med, Tianjin 300391, Peoples R China

关键词： fatty liver syndrome; RNA-seq; GSEA; self-constructed gene set; duck

期刊名称：POULTRY SCIENCE （ 影响因子：3.8； 五年影响因子：4.1 ）

ISSN： 0032-5791

年卷期： 2024 年 103 卷 9 期

页码：

收录情况： SCI

摘要： High-laying ducks are often fed highenergy, nutritious feeds to maintain high productivity, which predisposes them to lipid metabolism disorders and the development of fatty liver syndrome (FLS), which seriously affects production performance and has a substantial economic impact on the poultry industry. Therefore, it is necessary to elucidate the mechanisms underlying the development of fatty liver syndrome. In this study, seven Shan Partridge ducks, each with fatty liver syndrome and normal laying ducks, were selected, and Hematoxylin Eosin staining (HE staining), Masson staining, and transcriptome sequencing were performed on liver tissue. In addition to exploring key genes and pathways using conventional analysis methods, we constructed the first Kyoto Encyclopedia of Genes and Genomes (KEGG) database-based predefined gene set containing 12,764 pathways and 16,836 genes and further performed gene set enrichment analysis (GSEA) on the liver transcriptome data. Finally, key nodes and biological processes were identified via the protein-protein interaction (PPI) network. The results showed that the liver in the FL group exhibited steatosis and fibrosis, and a total of 3,663 genes with upregulated expression versus 2,296 downregulated genes were screened by conventional analysis. GSEA analysis and PPI network analysis revealed that the liver in the FL group exhibited disruption of the mitochondrial electron transport chain, leading to decreased oxidative phosphorylation and the secretion of excessive proinflammatory factors amid the continuous accumulation of lipids. Under continuous chronic inflammation, cell cycle arrest triggers apoptosis, while fibrosis becomes more severe, and procarcinogenic genes are activated, leading to the continuous development and deterioration of the liver. In conclusion, the predefined gene set constructed in this study can be used for GSEA, and the identified hub genes provide useful reference data and a solid foundation for the study of the genetic regulatory mechanism of fatty liver syndrome in ducks.

  • 相关文献
作者其他论文 更多>>
置顶
购物车
反馈

© 京ICP备09089781号-29 主办单位：广东省农业科学院

学术资源： 中国农业科学院 农业专业知识服务系统 农科联盟资源共建共享平台 中国农业科技文献与信息服务平台 中国农业期刊集成服务平台