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Cytochrome P450 and Glutathione S-Transferase Confer Metabolic Resistance to SYP-14288 and Multi-Drug Resistance in Rhizoctonia solani

文献类型： 外文期刊

作者： Cheng, Xingkai 1 ; Dai, Tan 1 ; Hu, Zhihong 2 ; Cui, Tongshan 1 ; Wang, Weizhen 1 ; Han, Ping 2 ; Hu, Maolin 3 ; Hao, Jianjun 4 ; Liu, Pengfei 1 ; Liu, Xili 1 ;

作者机构： 1.China Agr Univ, Dept Plant Pathol, Beijing, Peoples R China

2.Beijing Acad Agr & Forestry Sci, Inst Qual Stand & TestingTechnol, Beijing, Peoples R China

3.Shenzhen Agr Technol Promot Ctr, Shenzhen, Peoples R China

4.Univ Maine, Sch Food & Agr, Orono, ME USA

关键词： uncoupler; metabolic resistance; multi-drug resistance; Rhizoctonia solani; cytochrome P450s; glutathione-S-transferas

期刊名称：FRONTIERS IN MICROBIOLOGY （ 影响因子：6.064； 五年影响因子：6.843 ）

ISSN：

年卷期： 2022 年 13 卷

页码：

收录情况： SCI

摘要： SYP-14288 is a fungicide as an uncoupler of oxidative phosphorylation, which is effective in controlling fungal pathogens like Rhizoctonia solani. To determine whether R. solani can develop SYP-14288 resistance and possibly multi-drug resistance (MDR), an SYP-14288-resistant mutant of R. solani X19-7 was generated from wild-type strain X19, and the mechanism of resistance was studied through metabolic and genetic assays. From metabolites of R. solani treated with SYP-14288, three compounds including M1, M2, and M3 were identified according to UPLC-MS/MS analysis, and M1 accumulated faster than M2 and M3 in X19-7. When X19-7 was treated by glutathione-S-transferase (GST) inhibitor diethyl maleate (DEM) and SYP-14288 together, or by DEM plus one of tested fungicides that have different modes of action, a synergistic activity of resistance occurred, implying that GSTs promoted metabolic resistance against SYP-14288 and therefore led to MDR. By comparing RNA sequences between X19-7 and X19, six cytochrome P450s (P450s) and two GST genes were selected as a target, which showed a higher expression in X19-7 than X19 both before and after the exposure to SYP-14288. Furthermore, heterologous expression of P450 and GST genes in yeast was conducted to confirm genes involved in metabolic resistance. In results, the P450 gene AG1IA_05136 and GST gene AG1IA_07383 were related to fungal resistance to multiple fungicides including SYP-14288, fluazinam, chlorothalonil, and difenoconazole. It was the first report that metabolic resistance of R. solani to uncouplers was associated with P450 and GST genes.

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