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BrWAX3, Encoding a beta-ketoacyl-CoA Synthase, Plays an Essential Role in Cuticular Wax Biosynthesis in Chinese Cabbage

文献类型： 外文期刊

作者： Yang, Shuangjuan 1 ; Tang, Hao 1 ; Wei, Xiaochun 1 ; Zhao, Yanyan 1 ; Wang, Zhiyong 1 ; Su, Henan 1 ; Niu, Liujing 1 ; Yuan, Yuxiang 1 ; Zhang, Xiaowei 1 ;

作者机构： 1.Henan Acad Agr Sci, Inst Hort, Zhengzhou 450002, Peoples R China

2.Henan Inst Sci & Technol, Coll Hort & Landscape, Xinxiang 453003, Henan, Peoples R China

关键词： Brassica rapa; beta-ketoacyl-CoA synthase; wax synthesis; map-based cloning

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：6.208； 五年影响因子：6.628 ）

ISSN：

年卷期： 2022 年 23 卷 18 期

页码：

收录情况： SCI

摘要： In this study, we identified a novel glossy mutant from Chinese cabbage, named SD369, and all wax monomers longer than 26 carbons were significantly decreased. Inheritance analysis revealed that the glossy trait of SD369 was controlled by a single recessive locus, BrWAX3. We fine-mapped the BrWAX3 locus to an interval of 161.82 kb on chromosome A09. According to the annotated genome of Brassica rapa, Bra024749 (BrCER60.A09), encoding a beta-ketoacyl-CoA synthase, was identified as the candidate gene. Expression analysis showed that BrCER60.A09 was significantly downregulated in all aerial organs of glossy plants. Subcellular localization indicated that the BrCER60.A09 protein functions in the endoplasmic reticulum. A 5567-bp insertion was identified in exon 1 of BrCER60.A09 in SD369, which lead to a premature stop codon, thus causing a loss of function of the BrCER60.A09 enzyme. Moreover, comparative transcriptome analysis revealed that the 'cutin, suberine, and wax biosynthesis' pathway was significantly enriched, and genes involved in this pathway were almost upregulated in glossy plants. Further, two functional markers, BrWAX3-InDel and BrWAX3-KASP1, were developed and validated. Overall, these results provide a new information for the cuticular wax biosynthesis and provide applicable markers for marker-assisted selection (MAS)-based breeding of Brassica rapa.

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