Integrated metabolome and transcriptome analyses revealing the effects of thermal stress on lipid metabolism in juvenile turbot Scophthalmus maximus
文献类型： 外文期刊
作者： Zhao, Tingting 1 ; Ma, Aijun 2 ; Yang, Shuangshuang 2 ; Huang, Zhihui 2 ;
作者机构： 1.Shanghai Ocean Univ, Coll Fisheries & Life Sci, Minist Educ, Shanghai 201306, Peoples R China
2.Chinese Acad Fishery Sci, Yellow Sea Fisheries Res Inst, Shandong Key Lab Marine Fisheries Biotechnol & Ge, Qingdao Key Lab Marine Fish Breeding & Biotechnol, Qingdao 266071, Peoples R China
3.Pilot Natl Lab Marine Sci & Technol Qingdao, Lab Marine Biol & Biotechnol, Qingdao 266071, Peoples R China
关键词： Scophthalmus maximus; Metabolome; Transcriptome; Heat stress; Lipid metabolism
期刊名称：JOURNAL OF THERMAL BIOLOGY （ 影响因子：2.902； 五年影响因子：3.102 ）
ISSN： 0306-4565
年卷期： 2021 年 99 卷
页码：
收录情况： SCI
摘要： To gain insights into the influence of heat stress on lipid metabolism in juvenile turbot (Scophthalmus maximus), we analyzed the correlations between data obtained by transcriptome sequencing and metabolome sequencing of the kidney under different high temperature stimuli (20 degrees C, 25 degrees C and 28 degrees C) and control conditions (14 degrees C). We identified the differentially expressed genes and metabolites, which were found to be enriched in seven pathways (steroid hormone biosynthesis, primary bile acid biosynthesis, glycerophospholipid metabolism, linoleic acid metabolism, sphingolipid metabolism, glycerolipid metabolism and biosynthesis of unsaturated fatty acids) associated with lipid metabolism, according to KEGG pathway analysis. After correlation analysis of these differentially expressed genes, the most representative genes (lpcat2, Etnk1, TAZ, SCP2, ch25hl and gpd1l) and metabolites (citicoline, UPD-6-sulfoquinovose, dihydroxyacetone, taurine and o-phosphocholine) were selected according to their correlation coefficients. These genes and metabolites were found to be the key points to regulate lipid deposition and maintain lipid homeostasis through varying degrees of up-regulation or downregulation under heat stress, so as to relieve the disorder of lipid metabolism caused by heat stress, which is of great significance for breeding new heat-resistant varieties of turbot and provides a reliable theoretical basis for optimizing actual production. These results provide new clues for understanding the roles of lipid metabolism in fish under heat stress.
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