文献类型： 外文期刊

作者： Song, Fang ¹ ; Ji, Chuanya ² ; Wang, Tingting ³ ; Zhang, Zelu ⁴ ; Duan, Yaoyuan ³ ; Yu, Miao ³ ; Song, Xin ¹ ; Jiang, Yingchun ¹ ; He, Ligang ¹ ; Wang, Zhijing ¹ ; Ma, Xiaofang ¹ ; Zhang, Yu ² ; Pan, Zhiyong ³ ; Wu, Liming ¹ ;

作者机构： 1.Hubei Acad Agr Sci, Inst Fruit & Tea, Hubei Key Lab Germplasm Innovat & Utilizat Fruit T, Wuhan 430064, Peoples R China

2.Univ Sci & Technol China, Sch Life Sci, Div Life Sci & Med, Minist Educ Key Lab Cellular Dynam, Hefei 230027, Peoples R China

3.Huazhong Agr Univ, Coll Hort & Forestry Sci, Wuhan 430070, Peoples R China

4.Inner Mongolia Agr Univ, Coll Agr, Hohhot 010000, Peoples R China

关键词： Arbuscular mycorrhizal fungi; GRAS; molecular research; Poncirus trifoliata; protein interaction

期刊名称：INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES （ 影响因子：4.9； 五年影响因子：5.7 ）

ISSN： 1661-6596

年卷期： 2025 年 26 卷 5 期

页码：

收录情况： SCI

摘要： Arbuscular mycorrhizal (AM) fungi establish mutualistic symbiosis with most land plants, facilitating mineral nutrient uptake in exchange for photosynthates. As one of the most commercially used rootstocks in citrus, Poncirus trifoliata heavily depends on AM fungi for nutrient absorption. The GRAS gene family plays essential roles in plant growth and development, signaling transduction, and responses to biotic and abiotic stresses. However, the identification and functional characterization of GRAS family genes in P. trifoliata remains largely unexplored. In this study, a comprehensive genome-wide analysis of PtGRAS family genes was conducted, including their identification, physicochemical properties, phylogenetic relationships, gene structures, conserved domains, chromosome localization, and collinear relationships. Additionally, the expression profiles and protein interaction of these genes under AM symbiosis were systematically investigated. As a result, 41 GRAS genes were identified in the P. trifoliata genome, and classified into nine distinct clades. Collinearity analysis revealed seven segmental duplications but no tandem duplications, suggesting that segmental duplication played a more important role in the expansion of the PtGRAS gene family compared to tandem duplication. Additionally, 18 PtGRAS genes were differentially expressed in response to AM symbiosis, including orthologs of RAD1, RAM1, and DELLA3 in P. trifoliata. Yeast two-hybrid (Y2H) screening further revealed that PtGRAS6 and PtGRAS20 interacted with both PtGRAS12 and PtGRAS18, respectively. The interactions were subsequently validated through bimolecular fluorescence complementation (BiFC) assays. These findings underscored the crucial role of GRAS genes in AM symbiosis in P. trifoliata, and provided valuable candidate genes for improving nutrient uptake and stress resistance in citrus rootstocks through molecular breeding approaches.